Hanna Instruments hi 3897 User Manual

SPECIFICATIONS

Instruction Manual

www.hannainst.com

Dear Customer,
Thank you for choosing a Hanna Product. Please read the
instructions carefully before using the chemical test kit. It
will provide you with the necessary information for correct
use of the kit.
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable damage,
notify your Dealer or the nearest Hanna office immediately.
Each kit is supplied with:
• 1 HI 180IMD magnetic stirrer;
• HI 3897-0 Titrant Solution, 1 bottle (20 mL);
• Organic Solvent, 6 bottles (40 mL each) with one

magnetic bar each;

• 1 calibrated syringe of 5.0 ml;
• 1 calibrated syringe of 1.0 ml with tip.

Note:

Note: Any damaged or defective item must be returned in

its original packing materials.

Range

0 to 1% acidity

Smallest Increment

0.01 ml = 0.01 %

Analysis Method

Titration

Sample Size

4.6 mL (or 4.0 g)

Number of Tests

Case Dimensions

112 x 390 x 318 mm

Shipping Weight

3000 g

HI 3897

Extra Virgin

Olive Oil Acidity

TTTTTest Kit

est Kit

ISTR3897R2 07/04

The oil sample is firstly dissolved in an organic solvent
reagent (ethanol/ether); then it is titrated with a basic
solution (OH

). The final point of the titration is indicated by

the change in color of the solution, which turns from
yellow/green of the oil to pink.

• Remove the cap from 1 bottle of Organic Solvent

reagent. Place the bottle on the magnetic stirrer and
start a gentle stirring.

• Using the 1.0 ml syringe with tip, add the HI 3897-0

Titrant Solution reagent dropwise. Continue adding the
Titratant Solution reagent until the solution in the
bottle changes from colorless to a persistent light pink.
Replace the cap.
Note

Note

Note: stop adding the HI 3897-0 Titrant Solution
reagent to the bottle as soon as the solution is persistently
light pink colored.

• Take 4.6 ml of oil sample by use of the 5.0 ml syringe.

Alternatively, weight about 4.0 g (“w” grams) of oil by
use of an analytical balance.
Note

Note

Note: to dose exactly 4.6 ml of the oil sample:
(a) push the plunger completely into the syringe,
(b) insert the tip into the oil sample,
(c) pull the plunger up until the lower edge of the seal

is exactly on the 5 mL mark,

(d) take out the syringe and clean the outside of the

syringe tip. Be sure that no drops are hanging on
the tip of the syringe, if so eliminate them,

(e) then, keeping the syringe in vertical position above

the oil sample, push the plunger down into the
syringe, till the 4.6 ml sign,

(e) be sure that no drops are hanging on the tip of

the syringe, if so eliminate them,

(g) then, keeping the syringe in vertical position above

the (open) bottle of Organic Solvent, push the
plunger completely down into the syringe. Now the
exact amount of 4.6 mL has been added to the
bottle.

Specifications

Significance and Use

Chemical Reaction

Instructions

Acidity defined as oleic acid, is a parameter that indicates
the oil freshness: a high acidity value shows the oil is
becoming rancid, thus the oil quality is decreasing. According
to CEE 2568/91

CEE 2568/91

CEE 2568/91 regulation, olive oil is called extra virgin

when its acidity is below 1%: see table 1.
Acidity expressed in oleic acid is used to discriminate extra
virgin olive oil from other olive oils. It indicates an extraction
process made soon after the olive harvesting and with
natural, non chemical methods.

CAUTION!
Carefully open the bottle of the Organic Solvent
reagent because it may be under pressure!

READ ALL INSTRUCTIONS BEFORE USING THE TEST KIT

Table 1

• Remove the cap and add the oil sample to the Organic

Solvent bottle. Replace the cap.

• Place the sample on the magnetic stirrer and stir gently

until all the oil sample is dissolved in the Organic
Solvent reagent.

• Using the 1.0 ml graduated syringe with tip, take

1.0 ml of HI 3897-0 Titrant Solution reagent.
Note

Note

Note: to dose exactly 1.0 ml of the Titrant Solution
reagent:
(a) push the plunger completely into the syringe,
(b) insert tip into HI 3897-0 Titrant Solution reagent,
(c) pull the plunger out until the lower edge of the

plunger seal is on the 0 mL mark of the syringe,

(d) take out the syringe and clean the outside of the

syringe tip. Be sure that no drops are hanging on
the tip of the syringe, if so eliminate them.