GE 50 reactions 79760 Manuale Utente
14
0.5pmol
1pmol
2pmol
8pmol
300 bases
150 bases
Figure 1. Excess template DNA can reduce sequence extension lengths. In cases where
2pmol or more template DNA are sequenced, the supply of nucleotides can be exhausted
before extensions reach suitable length for optimal sequencing. These sequences were
run using up to 16
2pmol or more template DNA are sequenced, the supply of nucleotides can be exhausted
before extensions reach suitable length for optimal sequencing. These sequences were
run using up to 16
µ
g (8pmol) of M13mp18 DNA template.