GE 100 reactions 79770 Benutzerhandbuch

2

Components of the Kit ....................................................................................... 3

Quality Control .................................................................................................... 4

Safety Warnings and Precautions .............................................................. 4, 26

Introduction ......................................................................................................... 5

Materials Not Supplied ....................................................................................... 8

Protocol ............................................................................................................... 9

Supplementary Information ............................................................................. 11

General guidelines ......................................................................................... 11

Preparation of template DNA ......................................................................... 12

Cycle conditions and template quantity ......................................................... 12

Cycling temperatures ..................................................................................... 12

Number of cycles and quantity of template ................................................... 13

Designing a new sequencing primer ............................................................. 13

Sequencing PCR products ............................................................................ 15

Elimination of compressions .......................................................................... 15

Reading farther from or closer to the primer ................................................. 17

Denaturing gel electrophoresis ...................................................................... 18

Troubleshooting ............................................................................................... 20

Control DNA Sequence .................................................................................... 22

References ........................................................................................................ 23

Related Products .............................................................................................. 24

Contact Information ......................................................................................... 25