Nikon 44104 Benutzerhandbuch
Disc Diaphragm
c
Diaphragm
Condenser
Figure 2a
Viewing a Specimen
Carefully place a specimen slide under the spring loaded stage clips (8) and center the specimen directly over the hole in the
center of the stage (5).
You are now ready to focus and view the specimen, but first you must take some precautions so as not to damage the
specimen slide or objective lens. Your microscope is equipped with a safety rack stop (9) which allows you to regulate the
range of travel on the focuser. This assures that the objective lens will not accidentally come into contact with the
specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focus mechanism:
center of the stage (5).
You are now ready to focus and view the specimen, but first you must take some precautions so as not to damage the
specimen slide or objective lens. Your microscope is equipped with a safety rack stop (9) which allows you to regulate the
range of travel on the focuser. This assures that the objective lens will not accidentally come into contact with the
specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focus mechanism:
1. Raise the objective lens away from the specimen stage (5) by rotating the coarse focus knob (3) backwards
(clockwise).
2. Unthread the safety rack stop screw (9) about 2/3 of the way.
3. Turn the nosepiece (12) until the 40x lens is over the specimen.
4. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to
3. Turn the nosepiece (12) until the 40x lens is over the specimen.
4. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to
touch the objective lens to the slide).
5. Thread the safety screw (9) upward until it stops against the focus mechanism.
6. Rotate the knurled locking nut upward to lock the safety screw in place.
6. Rotate the knurled locking nut upward to lock the safety screw in place.
Focusing
Now that the specimen slide is placed directly under the objective lens, use the coarse focus knob (3) to focus on the
specimen.
specimen.
1. Always start with the lowest power (4x objective lens) and switch to higher power by rotating the nosepiece (12) to
the 10x and then 40x objective lenses. Be cautious not to let the objective lens touch the specimen slide when
changing to higher power.
changing to higher power.
2. Use the fine focus knob (11) to change the depth of field and obtain precise focus on the specimen you are observing.
3. If you run out of downward travel before reaching sharp focus, you can unscrew the safety screw (9) to allow for
3. If you run out of downward travel before reaching sharp focus, you can unscrew the safety screw (9) to allow for
more downward travel. Once again, be careful not to let the objective lens touch the specimen slide.
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