Gardner LCM IV User Manual

After all scan parameters have been selected, the instrument 
must then be zeroed. Afterward the sample can be analyzed.

Insert the zero solution cuvette into the cuvette compartment 
and close the cuvette compartment cover. 

Press Zero. 

The blank value is displayed on the screen.

Insert the analysis cuvette into the cuvette compartment and 
close the cuvette compartment cover. 

Press Read.  

The instrument will begin collecting Time Course data (kinetic 
data).

 Press the Mark button to mark the next measurement 

point visibly on the screen. This mark is not used by the 
instrument, but is available for the user and may indicate a 
significant event, such  as the addition of a sample or other 
reagent. The mark is also shown in the table.

Select Stop to stop taking sample readings.

After the Time Course data has been collected, the following 
manipulations can be done on the graphic data:

The Time Course Program is complete, if 

the instruments beeps after completion of the overall time

the graph is shown full-size,

the scaling of the x-axis occurs automatically,

the Cursor functions in the vertical navigation bar are 
highlighted.

After a time scan has been completed, the time and the 
absorbance/transmittance data is displayed as a curve.

Where the cursor is positioned on the curve, the elapsed time up 
to this point and the corresponding absorbance are highlighted.