Leica NCL-TH Leaflet

Leica Biosystems Newcastle Ltd

Balliol Business Park West

Benton Lane

Newcastle Upon Tyne NE12 8EW

United Kingdom
(

+44 191 215 4242

Novocastra

Lyophilized

Mouse Monoclonal Antibody

Tyrosine Hydroxylase

Product Code: NCL-TH

Intended Use

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Specificity

Human tyrosine hydroxylase. Also reacts with mouse and rat tyrosine hydroxylase.

Clone

1B5

Ig Class

1gG2a, kappa

Antigen Used for

Immunizations

Prokaryotic recombinant protein corresponding to a portion of the carboxyl terminal end of the

mouse tyrosine hydroxylase molecule.

Hybridoma Partner

Mouse myeloma (p3-NS1-Ag4-1).

Preparation

Lyophilized tissue culture supernatant containing sodium azide.

Reconstitute with 1 mL or 0.1 mL of sterile distilled water as indicated on vial label.

Effective on Frozen Tissue

Not evaluated.

Effective on Paraffin Wax

Embedded Tissue

Yes.

Recommendations on Use

Immunohistochemistry on paraffin sections.
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra

Epitope Retrieval Solution pH 6.
Suggested dilution: 1:40 for 30 minutes at 25 °C. This is provided as a guide and users should

determine their own optimal working dilutions.
Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.

For further product information or support, contact your local distributor or regional office of Leica

Biosystems, or alternatively, visit the Leica Biosystems Web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining

systems or automated platforms.

Positive Controls

Immunohistochemistry: brain.
Western Blotting: Mouse brain.

Staining Pattern

Cytoplasmic; Dopaminergic cells.

Storage and Stability

Store unopened lyophilized antibody at 2-8

C. Under these conditions, there is no significant

loss in product performance up to the expiry date indicated on the vial label. The reconstituted

antibody is stable for at least two months when stored at 2-8

C. For long term storage, it is

recommended that aliquots of the antibody are frozen at -20

C (frost-free freezers are not

recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on

the day of use.

Warnings and Precautions

This reagent has been prepared from the supernatant of cell culture. As it is a biological product,

reasonable care should be taken when handling it. This reagent contains sodium azide. A Material

Safety Data Sheet is available upon request or available from www.LeicaBiosystems.com