Hanna Instruments hi 38081 User Manual

Instruction Manual

www.hannainst.com

Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instruction sheet carefully before using the

test kit. It will provide you with the necessary information

for correct use of the kit. If you need additional information,

do not hesitate to e-mail us at tech@hannainst.com.
Remove the chemical test kit from the packing material and

examine it carefully to make sure that no damage has

occurred during shipping. If there is any noticeable dam-

age, notify your Dealer or the nearest Hanna office

immediately.
Each kit is supplied with:

• HI 38081A-0 Ca & Mg Reagent, 1 bottle with

dropper (30 mL);

• HI 38081B-0 EDTA Solution, 2 bottles (2x120 mL);
• Calmagite Indicator, 1 bottle with dropper (10 mL);

• Demineralizer Bottle with filter cap for about 12 liters

of deionized water (depending on the hardness level

of water to be treated);

• 1 calibrated plastic vessel (50 mL);
• 1 plastic pipette (1 mL);
• 1 plastic pipette (3 mL);
• 1 syringe (1 mL) with tip.

Note: Any damaged or defective item must be returned in

its original packing materials.

HI 38081

Calcium and

Magnesium

Test Kit for

Irrigation Water

ISTR38081 02/00 PRINTED IN ITALY

Calcium presence in natural waters results from passage

through deposits of limestone, dolomite, gypsum and gypsif-

erous shale. The calcium concentration in water supplies

may extend from 0 to several hundred milligrams per liter,

depending on its source and treatment.
Magnesium is a common constituent of natural waters; in

concentrations greater than 125 mg/L it can cause diuretic

effect. It is also an important contributor to the hardness of

water: when heated, magnesium salts break down forming

incrustation in boilers. Moreover, magnesium is necessary to

plant metabolism since it is an essential constituent of

organic molecules such as chlorophyll.

SPECIFICATIONS

SIGNIFICANCE AND USE

CHEMICAL REACTION

READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT

1- Remove the cap and fill the

Demineralizer Bottle with tap

water.

INSTRUCTIONS

The Hanna Test Kit determines Calcium and Magnesium in

irrigation water via a titrimetric method: the indicator

chelates with the ions Calcium and Magnesium to form a

red colored complex. As EDTA is added, calcium and magne-

sium complex with it: the reaction end point is indicated by

a change in color of the indicator from red to blue.
Note: meq/L is milliequivalent per liter.

2- Replace the cap

and shake for at

least 2 minutes. The

demineralized water

is now ready.

3- Using the 3 mL plastic pipette, transfer 3

mL of your water sample to the 50 mL

plastic vessel.

4- Flip open the top of the Dem-

ineralizer Bottle cap. Squeeze

the bottle gently to add dem-

ineralized water to the plastic

vessel up to the 25 mL mark.

5- Add 5 drops of

HI 38081A-0 Ca

& Mg Reagent

and swirl to mix.

6- Add 1 drop of

Calmagite Indica-

tor and swirl to

mix. If calcium

and magnesium

are present, the

solution will turn

wine red.

7- Take the syringe and push

the plunger completely down

into the syringe. Insert tip

into the HI 38081B-0 EDTA

solution and pull the plunger

out until the lower edge of

the seal is on the 0 mark of

the syringe.

8- Slowly add the titration solution drop by drop, swirling

after each drop.

Range

> 0 meq/L as Ca & Mg

Smallest Increment

0.6 meq/L for 1.0 mL sample
0.2 meq/L for 3.0 mL sample

Analysis Method

Titration

Sample Size

1.0 mL or 3.0 mL

Number of Tests

100 (average)

Case Dimensions

235x175x115 mm (9.2x6.9x4.5")

Shipping Weight

671 g (23.7 oz.)

9- As the color

changes from pink

to purple, swirl for

15 seconds after

each additional

drop. Repeat this

until the solution

turns pure blue.

Read off the mil-

liliters of titration solution used from the syringe.

10- Calculate the meq/L of Calcium and Magnesium in your

sample as follows:

meq/L (Ca + Mg) = mL of titrant x 20

11- If your sample requires more than 1 mL of titrant to

turn pure blue it requires dilution. Repeat the test by

using the 1 mL pipette to transfer 1 mL of your water

sample to the plastic vessel.

12- Follow the instructions from step 4 to step 9.
13- Calculate the meq/L of Calcium and Magnesium in your

sample as follows:

meq/L (Ca + Mg) = mL of titrant x 60

14- To convert the reading in mg/L (ppm, parts per million)

of CaCO

, multiply the meq/L by 50.

15- Rinse all labware with demineralized water after each

analysis and shake dry.

Note: High amounts of copper in your sample will alter the

final end point color. The solution will change from wine

red to purple without turning pure blue. In this case

add drops of titrant until no visible change in color is

obtained.

The chemicals contained in this kit may be hazardous if
improperly handled. Read Health and Safety Data Sheet
before performing this test.

HEALTH AND SAFETY

REFERENCES

Adaptation of Standard Methods for the Examination of
Water and Wastewater

, 18

edition, 1992, APA AWWA WEF.