Leica CM3050 S User Manual
13
Leica CM3050 S – Cryostat
2.3.4 Handling microtome knives/blades
•
Danger!
--> Microtome knives and disposable blades
--> Microtome knives and disposable blades
have extremely sharp cutting edges and
can cause serious injuries. Therefore:
can cause serious injuries. Therefore:
•
Handle knives / blades with utmost care.
•
Never leave any knives / blades in unpro-
tected places!
tected places!
•
Never place a knife, no matter where,
with the cutting edge facing upwards.
with the cutting edge facing upwards.
•
Never try to catch a falling knife.
•
Always insert the specimen before
inserting the knife.
inserting the knife.
2.3.5 Knife guard/handwheel lock
•
Always cover the cutting edge with the knife
guard and lock the handwheel:
•
guard and lock the handwheel:
•
Prior to doing any work on knife and / or
specimen.
specimen.
•
Prior to exchanging specimens.
•
During work breaks.
2.3.6 Motorized sectioning
•
Do not interrupt sectioning / trimming by set-
ting the sliding potentiometer to zero speed!
--> This does not really switch the sectioning
ting the sliding potentiometer to zero speed!
--> This does not really switch the sectioning
function off - it only operates at ‘0’ speed.
If the sliding potentiometer is accidental-
ly moved, the instrument will resume sec-
tioning immediately (risk of injury)!
If the sliding potentiometer is accidental-
ly moved, the instrument will resume sec-
tioning immediately (risk of injury)!
•
During motorized sectioning, always center
the handwheel grip!
the handwheel grip!
2.3.7 Defrosting/Handling frozen tissue
•
Never leave specimens unattended in the
cryochamber over an extended period of
time!
--> In case of power failure or instrument
failure, or during the automatic defrost cy-
cle, tissue material can be destroyed.
cryochamber over an extended period of
time!
--> In case of power failure or instrument
failure, or during the automatic defrost cy-
cle, tissue material can be destroyed.
•
During the defrost cycle the cryochamber is
partially warmed. - Therefore:
•
partially warmed. - Therefore:
•
Remove sensitive specimens from the
chamber prior to defrosting.
chamber prior to defrosting.
•
If automatic defrosting is programmed to
take place during the night, remember to
remove all specimens from the cryo-
chamber prior to leaving work.
take place during the night, remember to
remove all specimens from the cryo-
chamber prior to leaving work.
2.3.8 Frozen parts of the instrument and
frozen accessories
•
Prolonged contact of bare skin to frozen
surfaces of the instrument or to frozen
accessories (specimen discs, knife holder,
shelves etc.) can cause frostbite.
•
surfaces of the instrument or to frozen
accessories (specimen discs, knife holder,
shelves etc.) can cause frostbite.
•
Wear protective gloves.
2.
Safety