Leica CM3050 S User Manual
45
Leica CM 3050 S – Cryostat
H O M E
5.7
Defrosting
The Leica CM3050 S has 3 (instruments without
specimen cooling: 2) different defrosting options.
specimen cooling: 2) different defrosting options.
-
Automatic chamber defrost cycle
-
Manual chamber defrost cycle
-
Specimen head defrosting (manual) - instru-
ments with specimen cooling only.
ments with specimen cooling only.
Read the safety instructions in chap-
ter 2.3.7 ‘Defrosting / frozen speci-
mens’ !
ter 2.3.7 ‘Defrosting / frozen speci-
mens’ !
Make sure to remove sensitive speci-
mens from the cryochamber before
the defrost cycle starts!
mens from the cryochamber before
the defrost cycle starts!
5.7.1 Automatic chamber defrost cycle
The automatic defrost cycle is carried out once
every 24 hours at the preselected time.
(For details see chapter 5.3.4 ‘Menu functions’ -
items 6 and 7).
every 24 hours at the preselected time.
(For details see chapter 5.3.4 ‘Menu functions’ -
items 6 and 7).
During the defrost cycle, the corresponding as-
terisk is displayed inversely:
terisk is displayed inversely:
Once the defrost cycle is finished, the instrument
automatically switches back to refrigeration.
automatically switches back to refrigeration.
Instruments with specimen cooling:
When defrosting starts, to avoid icing the speci-
men head is automatically adjusted to a temper-
ature between -10 °C and -5 °C, i.e. the set tem-
perature value is deactivated temporarily.
men head is automatically adjusted to a temper-
ature between -10 °C and -5 °C, i.e. the set tem-
perature value is deactivated temporarily.
5.
Operation
The specimen head refrigeration is automatical-
ly switched back on when:
•
ly switched back on when:
•
at least 4 hours have passed since the de-
frost cycle was completed;
frost cycle was completed;
•
and the actual chamber temperature devi-
ates less than 5 K from set value.
ates less than 5 K from set value.
After the specimen head refrigeration has been
switched back on, the specimen head tempera-
ture rises briefly to +10 °C and then decreases
until it reaches the set value.
switched back on, the specimen head tempera-
ture rises briefly to +10 °C and then decreases
until it reaches the set value.
Switching specimen cooling back on immedi-
ately after defrosting:
As soon as the chamber defrost cycle is com-
pleted, specimen cooling can be switched back
on immediately:
ately after defrosting:
As soon as the chamber defrost cycle is com-
pleted, specimen cooling can be switched back
on immediately:
•
Press
until menu item ‘Set specimen
temperature’ is displayed:
•
Press
.
Specimen cooling is switched back on.
Despite the possibility of switching
the specimen cooling system back
on immediately after the automatic
chamber defrost cycle, we recom-
mend the automatic defrost cycle
be set at a point in time when the
entire procedure can be controlled
automatically.
(See also information on menu
items 6 and 7 in chapter 5.3.4 ‘Menu
functions’).
the specimen cooling system back
on immediately after the automatic
chamber defrost cycle, we recom-
mend the automatic defrost cycle
be set at a point in time when the
entire procedure can be controlled
automatically.
(See also information on menu
items 6 and 7 in chapter 5.3.4 ‘Menu
functions’).
C T - 3 0 ° C
O T - 3 5 ° C
S E T
T E M P
O T - 3 5 ° C
S E T
T E M P
O T - 3 5 ° C
-