Leica DM4000B User Manual
57
8. Imaging Procedure for Leica DM4000 B/DM5000 B
8.2 Fluorescence
• Switch to the fluorescent light axis (FLUO) by
pushing the TL/IL button.
• Insert a specimen and rotate a suitable objec-
tive into place.
• The current fluorescence cube is indicated on
the display.
• Closing the incident light shutter protects your
specimen from fading.
Do so by pressing the SHUTTER variable key.
(For key occupation please see “Identification
Sheet”.)
Do so by pressing the SHUTTER variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates the symbol:
↓
• Selecting the fluorescence filter cube:
Press the variable keys
Cube or Cube
Cube or Cube
• The fluorescence intensity can be increased
using the Booster Lens on the right side of the
stand (Fig. 69).
stand (Fig. 69).
• For multifluorescence, use of a Excitation
Manager is recommended. The Excitation
Manager is inserted into the right side of the
stand up to the last stop (Fig. 70).
Manager is inserted into the right side of the
stand up to the last stop (Fig. 70).
• Using Booster Lens and Excitation Manager,
the Excitation Manager can be inserted into
the back receptacle.
the back receptacle.
Fig. 70
Inserting the Excitation Manager
Fig. 69
Inserting the Booster Lens
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