GE 500 reactions User Manuals

Table of Contents

• Components of the Kit

  3
• Quality Control

  4
• Introduction

  5
• Materials Not Supplied

  8
• Protocol

  9
• Supplementary Information

  11
• General guidelines

  11
• Preparation of template DNA

  12
• Cycle conditions and template quantity

  12
• Cycling temperatures

  12
• Number of cycles and quantity of template

  13
• Designing a new sequencing primer

  13
• Sequencing PCR products

  15
• Elimination of compressions

  15
• Reading farther from or closer to the primer

  17
• Denaturing gel electrophoresis

  18
• Troubleshooting

  20
• Control DNA Sequence

  22
• References

  23
• Related Products

  24
• Contact Information

  25