Leica B523 User Manuals

Table of Contents

• GENERAL USER INFORMATION

  1
• SETTINGS FOR TRANSMITTED LIGHT MICROSCOPY

  1
• Turning on the microscope

  1
• XY-stage

  1
• Placing the sample

  2
• Objectives

  2
• Focusing

  3
• Eye pieces (oculars)

  4
• Dual viewing attachment

  4
• Koehler illumination

  6
• Polarization contrast

  8
• Filters

  8
• Dark-field microscopy

  9
• Turning off the microscope

  9
• IMAGE ACQUISITION

  10
• Accessing the computer

  10
• View Finder application

  10
• Adjustments before image acquisition

  10
• Capture resolution

  10
• Sensitivity

  10
• Exposure Mode

  11
• White balance

  11
• Levels adjustment

  11
• Focus tool

  12
• Over/Underexposure

  12
• Averaging

  12
• Capturing the image

  13
• Viewing images

  13
• Post-imaging adjustments

  13
• Cropping the image

  13
• Scale bars

  14
• SAVING AND TRANSFERRING IMAGES

  14
• FINISHING YOUR IMAGING

  14
• TROUBLESHOOTING

  15
• There is no light

  15
• Light is too dark

  15
• Problems with focus or picture clarity

  15
• View is partial

  15
• Colors have red/yellow tint

  15
• Ocular view is not at the same focus as the camera view

  15
• Computer login fails

  16