Carl Zeiss LSM 710 Manual De Usuario

 
 

 
 
05/2008  

19 

The scanned image appears in a false-color 
presentation (Fig. 22). 
 
If the image is too bright, it appears red on the 
screen. Red = saturation (maximum). 
If the image is not bright enough, it appears blue 
on the screen. Blue = zero (minimum). 
 

•  Set the Pinhole to 1 Airy Unit (Fig. 23). 
•  Set the Detector Gain high. 
•  When the image is saturated, reduce AOTF 

transmission in the Laser control section of the 
Channels Tool  (Fig. 23) using the slider to 
reduce the intensity of the laser light to the 
specimen. 

 

•  Increase the Digital Offset until all blue pixels 

disappear, and then make it slightly positive 
(Fig. 23). 

•  Reduce the Master Gain until the red pixels 

only just disappear.