Leica DM4000B Manual De Usuario

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6. Startup
• Open the field diaphragm just enough for it to
disappear from the field of view (41d).
Caution:
Do not adjust the aperture diaphragm. The aper-
ture diaphragm is already set optimally for each
objective.
6.6. Checking Phase Contrast Rings
A
B
C
D
Fig. 41
Köhler Illumination
Field diaphragm not focused, not centered
Field diaphragm focused, but not centered
Field diaphragm focused and centered
Diameter is too small, however
Field diameter (light) = Field diameter (view)
(Köhler Illumination)
Fig. 40
Condenser centering
Centering bolts
1
1