Leica B523 Manual De Usuario
1
GENERAL USER INFORMATION .............................................................................. 1
2
SETTINGS FOR TRANSMITTED LIGHT MICROSCOPY...................................... 1
2.1
Turning on the microscope .................................................................................................... 1
2.2
XY-stage................................................................................................................................ 1
2.3
Placing the sample................................................................................................................. 2
2.4
Objectives.............................................................................................................................. 2
2.5
Focusing ................................................................................................................................ 3
2.6
Eye pieces (oculars)............................................................................................................... 4
2.7
Dual viewing attachment ....................................................................................................... 4
2.8
Koehler illumination.............................................................................................................. 6
2.9
Polarization contrast .............................................................................................................. 8
2.10
Filters..................................................................................................................................... 8
2.11
Dark-field microscopy........................................................................................................... 9
2.12
Turning off the microscope ................................................................................................... 9
3
IMAGE ACQUISITION ................................................................................................ 10
3.1
Accessing the computer....................................................................................................... 10
3.2
View Finder application ...................................................................................................... 10
3.3
Adjustments before image acquisition................................................................................. 10
3.3.1
Capture resolution........................................................................................................... 10
3.3.2
Sensitivity ....................................................................................................................... 10
3.3.3
Exposure Mode............................................................................................................... 11
3.3.4
White balance ................................................................................................................. 11
3.3.5
Levels adjustment ........................................................................................................... 11
3.3.6
Focus tool ....................................................................................................................... 12
3.3.7
Over/Underexposure....................................................................................................... 12
3.3.8
Averaging ....................................................................................................................... 12
3.4
Capturing the image ............................................................................................................ 13
3.5
Viewing images ................................................................................................................... 13
3.6
Post-imaging adjustments.................................................................................................... 13
3.6.1
Cropping the image......................................................................................................... 13
3.6.2
Scale bars........................................................................................................................ 14
4
SAVING AND TRANSFERRING IMAGES ............................................................... 14
5
FINISHING YOUR IMAGING..................................................................................... 14
6
TROUBLESHOOTING ................................................................................................. 15
6.1
There is no light................................................................................................................... 15
6.2
Light is too dark .................................................................................................................. 15
6.3
Problems with focus or picture clarity................................................................................. 15
6.4
View is partial ..................................................................................................................... 15
6.5
Colors have red/yellow tint ................................................................................................. 15
6.6
Ocular view is not at the same focus as the camera view .................................................... 15
6.7
Computer login fails ............................................................................................................ 16