Leica CM3050 S Manuel D’Utilisation
43
Leica CM 3050 S – Cryostat
5.
Operation
Inserting the knife
•
Insert the knife / disposable blade into the
knife holder.
knife holder.
•
Select appropriate clearance angle at knife
holder (settings between 4° and 6° are suit-
able for the majority of applications).
holder (settings between 4° and 6° are suit-
able for the majority of applications).
For details on how to insert and ad-
just the knives, please refer to the
separate instruction manual(s) for
your knife holder(s).
See also chapter 2.2 ‘Integrated
safety devices’ incl. subchapter
2.2.4 ‘Knife guard’.
just the knives, please refer to the
separate instruction manual(s) for
your knife holder(s).
See also chapter 2.2 ‘Integrated
safety devices’ incl. subchapter
2.2.4 ‘Knife guard’.
Manual trimming
•
Adjust knife / knife holder relative to speci-
men (see ‘Knife holder’ instruction manual).
men (see ‘Knife holder’ instruction manual).
•
Unlock handwheel.
•
Use motorized coarse feed to bring speci-
men close to knife (see chapter 5.4.5 ‘Coarse
feed functions’).
men close to knife (see chapter 5.4.5 ‘Coarse
feed functions’).
•
Select desired section thickness (see chap-
ter 5.4.7 ‘Setting the section thickness’).
ter 5.4.7 ‘Setting the section thickness’).
•
Move anti-roll plate away from knife.
•
Rotate handwheel to trim specimen down to
desired sectioning plane.
desired sectioning plane.
Motorized trimming
•
Adjust knife / knife holder relative to speci-
men (see ‘Knife holder’ instruction manual).
men (see ‘Knife holder’ instruction manual).
•
Unlock handwheel.
•
Center handwheel grip (See chapter 2.2.2).
•
Use motorized coarse feed to bring speci-
men close to knife (see chapter 5.4.5 ‘Coarse
feed functions’).
men close to knife (see chapter 5.4.5 ‘Coarse
feed functions’).
•
Move anti-roll plate away from knife.
--> Next steps: see chapter 5.4.6 ‘Trimming’.
Sectioning
•
Adjust knife / knife holder relative to speci-
men (see ‘Knife holder’ instruction manual).
men (see ‘Knife holder’ instruction manual).
•
Place anti-roll guide onto knife.
•
Orient specimen, if necessary (see chapter
5.5.5 ‘Specimen orientation’).
5.5.5 ‘Specimen orientation’).
•
Select desired section thickness (see chap-
ter 5.4.7 ‘Setting the section thickness’).
ter 5.4.7 ‘Setting the section thickness’).
•
Start sectioning at approx. 20 μm, reduce
section thickness gradually until reaching
the appropriate thickness for the type of
specimen to be sectioned.
section thickness gradually until reaching
the appropriate thickness for the type of
specimen to be sectioned.
•
Discard the first 2 to 3 sections cut at the de-
sired section thickness.
sired section thickness.
When sectioning manually, rotate the hand-
wheel evenly and at uniform speed.
wheel evenly and at uniform speed.
For all details on motorized sectioning, please
refer to chapters 5.4.1 to 5.4.4:
‘Selecting a sectioning mode / Start and stop
sectioning’, ‘Setting the sectioning window’,
‘Selecting sectioning speed’, ‘Emergency stop’.
refer to chapters 5.4.1 to 5.4.4:
‘Selecting a sectioning mode / Start and stop
sectioning’, ‘Setting the sectioning window’,
‘Selecting sectioning speed’, ‘Emergency stop’.
For advice on appropriate Sectioning tempera-
tures for different types of tissue, please refer to
chapter 6.3 ‘Reference chart for temperature
settings’.
tures for different types of tissue, please refer to
chapter 6.3 ‘Reference chart for temperature
settings’.
5.5.6 Trimming / sectioning