Leica CM3050 S Manuel D’Utilisation
5
Leica CM3050 S – Cryostat
Table of Contents
4.
Instrument Properties .............................................................................................................. 21
4.1
Overview ............................................................................................................................................ 21
4.2
Technical Data ........................................................................................................................... 23
5.
Operation ................................................................................................................................... 25
5.1
Operating the instrument for the first time ................................................................................... 25
5.2
Switching on/fuses ........................................................................................................................... 26
5.3
Control panel 1 .................................................................................................................................. 27
5.3.1
Key functions in control panel 1 ..................................................................................................... 27
5.3.2
Display functions in control panel 1 .............................................................................................. 27
5.3.3
Display indications when switching on ........................................................................................ 28
5.3.4
Menu functions: setting parameters of refrigeration, time and preset counter .................... 29
5.3.5
Status display .................................................................................................................................... 32
5.3.6
Indication of refrigeration state, section thickness setting and counters .............................. 33
5.4
Control panel 2 .................................................................................................................................. 34
5.4.1
Selecting a sectioning mode / ........................................................................................................ 35
5.4.2
Setting the sectioning window ....................................................................................................... 36
5.4.3
Selecting sectioning speed ............................................................................................................ 37
5.4.4
Emergency stop ................................................................................................................................ 37
5.4.5
Coarse feed keys .............................................................................................................................. 38
5.4.6
Trimming and sectioning functions ............................................................................................... 39
5.4.7
Setting the section thickness ......................................................................................................... 39
5.5
Daily operation of the instrument .................................................................................................. 40
5.5.1
Freezing specimens onto specimen .............................................................................................. 40
5.5.2
Freezing specimens onto specimen .............................................................................................. 41
5.5.3
Optional accessories for freezing .................................................................................................. 41
5.5.4
Inserting specimen discs into the .................................................................................................. 42
5.5.5
Specimen orientation ....................................................................................................................... 42
5.5.6
Trimming / sectioning ...................................................................................................................... 43
5.6
Finishing work ................................................................................................................................... 44
5.6.1
Daily routine steps ............................................................................................................................ 44
5.6.2
Switching off the instrument for a ................................................................................................. 44
5.7
Defrosting .......................................................................................................................................... 45
5.7.1
Automatic chamber defrost cycle ................................................................................................. 45
5.7.2
Manual chamber defrost cycle ...................................................................................................... 46
5.7.3 Defrosting the specimen head ................................................................................................ 46