Leica DM100 Manuel D’Utilisation

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Leica DM100 
User Manual 
30
Oil Immersion Technique (only 100× objective)
Oil Immersion Technique
1.  Search for the area on the specimen slide 
that you want to study.
2.  Move the specimen stage down to the 
lowest position using the coarse adjust-
ment knob.
3.  Add a drop of immersion oil from Leica 
to the area of the specimen slide you are 
studying. 
4.  Rotate the 100× objective to the working 
position.
5.  Slowly move the specimen stage upwards 
using the coarse adjustment knob until 
the oil drop on the slide comes into direct 
contact with the lens of the oil immersion 
objective. 
6.  Hold the knurled ring on the objective 
nosepiece and swing the objective back 
and forth to eliminate air bubbles. Then 
bring the oil objective into the final posi-
tion, so that the oil drop is between the 
front lens of the objective and the speci-
men slide.