Leica B523 Manuel D’Utilisation
1
1
General user information
Leica DM LB research microscope is only for the Molecular Cancer Biology (MCBP)
and Genome-scale Biology (GSB) research programs internal use. It is a transmitted
light microscope that cannot be used for fluorescence microscopy. Bright-field,
polarization contrast, and low-quality dark-field illumination are available. There are
no oil objectives available, thus the resolution of the objectives is lower than that of
the Axioplans. There is a Leica manual on a shelf near the microscope.
and Genome-scale Biology (GSB) research programs internal use. It is a transmitted
light microscope that cannot be used for fluorescence microscopy. Bright-field,
polarization contrast, and low-quality dark-field illumination are available. There are
no oil objectives available, thus the resolution of the objectives is lower than that of
the Axioplans. There is a Leica manual on a shelf near the microscope.
Reservations for the microscope are made through the Scheduler. Access to the
reservation system and instructions for its use are at MIU web page:
http://www.miu.helsinki.fi/reservations.htm. MIU website has information about the
instruments available, sample preparation, imaging techniques etc. Every user of
instruments maintained by MIU is expected to be familiar with the information on the
MIU user info web page: http://www.miu.helsinki.fi/userinfo.htm. In particular, if you
use any MIU instruments or services for your publication, you are expected to
acknowledge MIU.
reservation system and instructions for its use are at MIU web page:
http://www.miu.helsinki.fi/reservations.htm. MIU website has information about the
instruments available, sample preparation, imaging techniques etc. Every user of
instruments maintained by MIU is expected to be familiar with the information on the
MIU user info web page: http://www.miu.helsinki.fi/userinfo.htm. In particular, if you
use any MIU instruments or services for your publication, you are expected to
acknowledge MIU.
2
Settings for transmitted light microscopy
2.1
Turning on the microscope
Turn on the microscope by using a switch located on the left-hand side of the
microscope (Figure 1). This turns on the transmitted light source (halogen lamp). If
the light is too dim or bright this can be corrected by adjusting the brightness control
(Figure 1). For other light intensity problems, see the troubleshooting section on page
15.
microscope (Figure 1). This turns on the transmitted light source (halogen lamp). If
the light is too dim or bright this can be corrected by adjusting the brightness control
(Figure 1). For other light intensity problems, see the troubleshooting section on page
15.
Figure 1. Power switch (red circle) and brightness control for halogen lamp (red arrow)
2.2
XY-stage
Move the specimen stage in the XY-direction to center the sample or wanted detail in
the field of view. This is achieved by turning the two adjustment wheels connected to
the stage on the right-hand side (arrows in Figure 2). Upper wheel controls the
the field of view. This is achieved by turning the two adjustment wheels connected to
the stage on the right-hand side (arrows in Figure 2). Upper wheel controls the