Leica DM4500P Manuel D’Utilisation

44

Fig. 44a Phase contrast centering procedure
PH=phase contrast ring, LR=light ring
a

Condenser in bright field (BF) position

Condenser in phase contrast (PH) position
Light ring (LR) not centered

Light ring and phase ring centered

Focusing telescope

Adjustable eyelens

Clamping ring for fixing the focus position

1

2

If your microscope is equipped for the use of
phase contrast, the light rings that fit the objec-
tives are built into the condenser.
The light rings are already centered in the facto-
ry. However, the centering should be rechecked.

Every objective is assigned its own light ring in
the condenser disk. Therefore, a check must be
performed for each objective. When rotating in
an objective that is suitable for phase contrast,
the corresponding light ring is set automatically.

• Press the BF (bright field) button (one of the

variable function keys, to the left behind the
focus wheels).

• In the place of an eyepiece, insert the focus-

ing telescope (Fig. 43) into the observation
tube.

• Rotate the phase contrast objective with the

lowest magnification into place.

• Focus on the specimen with the focus wheel .

• Focus the ring structure (44a.a) by slightly

loosening the clamping ring (43.2) and moving
the eyelens (43.1).

• Retighten the clamping ring.

• Press the PH (Phase Contrast) button. The

ring diaphragm (light ring) in the condenser is
turned inward.

• If the light ring and the phase ring are not

shown as arranged in Fig. 44a.c, the light ring
must be centered.