Leica DM500 B Manuel D’Utilisation

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Leica DM 500, DM500 B 
Manual 
32
Oil Immersion Technique
Oil Immersion Technique
1.  Find the field of view on the slide that you 
would like to inspect.
2.  Lower the stage to its lowest position using 
the coarse focus knob.
3.  Place a drop of Leica Immersion Oil on the 
specimen slide on the area of the specimen 
that you were inspecting. 
4.  Swing the oil immersion objective in place 
(the objective will be labeled "OIL")
5.  Raise the stage slowly using the coarse 
focus knob until the drop of oil on the spec-
imen slide just comes in contact with the 
front of the oil immersion objective lens. 
6.  Grip the knurled nosepiece ring and rock 
the objective back and forth to eliminate 
any air bubbles, then settle the oil objec-
tive into position with the oil between the 
front lens of the objective and the speci-
men slide.