Leica M205 FA Manuel D’Utilisation
Leica M-series
User Manual
117
About Fluorescence Microscopy
Functional principle
Fluorescent substances light up when irradi-
ated with short-wave excitation light, and fluo-
rescence microscopy takes advantage of this
property. Specimens without self-fluorescence
are stained with a fluorescent substance. One
such dye is green fluorescent protein (GFP),
which is used in molecular biology.
ated with short-wave excitation light, and fluo-
rescence microscopy takes advantage of this
property. Specimens without self-fluorescence
are stained with a fluorescent substance. One
such dye is green fluorescent protein (GFP),
which is used in molecular biology.
The Leica M Series
The Leica M series fluorescence stereomicro-
scopes allow entire fluorescent specimens to
be studied, unprepared and in three dimen-
sions. The fully apochromatic optics system and
the enormous zoom range make the Leica M
series stereomicroscopes your first choice. The
patented TripleBeam® third beam path for fluo-
rescence illumination and the patented FLUOIII®
filter system generate highest-quality fluores-
cence images. The very bright high-pressure
mercury burner and specially selected filter sets
enable you to distinguish the finest structures
and enhance the quantity of information for
incident light fluorescence.
scopes allow entire fluorescent specimens to
be studied, unprepared and in three dimen-
sions. The fully apochromatic optics system and
the enormous zoom range make the Leica M
series stereomicroscopes your first choice. The
patented TripleBeam® third beam path for fluo-
rescence illumination and the patented FLUOIII®
filter system generate highest-quality fluores-
cence images. The very bright high-pressure
mercury burner and specially selected filter sets
enable you to distinguish the finest structures
and enhance the quantity of information for
incident light fluorescence.
The filter system
The FLUOIII® filter system consists of a rapid
filter changer for barrier and excitation filters
and a filter insert for a filter holder with your
choice of filter. A total of four filter sets (which
are labeled) can be used in the rapid filter
changer. An empty filter cartridge is also avail-
able for individual filter combinations. Each
filter set includes two barrier filters to the visual
beam paths and an excitation filter to the illu-
mination beam path.
filter changer for barrier and excitation filters
and a filter insert for a filter holder with your
choice of filter. A total of four filter sets (which
are labeled) can be used in the rapid filter
changer. An empty filter cartridge is also avail-
able for individual filter combinations. Each
filter set includes two barrier filters to the visual
beam paths and an excitation filter to the illu-
mination beam path.