Hanna Instruments hi 3864 ユーザーズマニュアル

Instruction Manual

www.hannainst.com

Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instructions carefully before using the
chemical test kit. It will provide you with the necessary
information for correct use of the kit.
Remove the chemical test kit from the packing material and

examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable dam-
age, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:

• HI 3864A-0 Reagent, packets (100 pcs);
• HI 3864B-0 Reagent, packets (100 pcs);
• 1 checker disc (with 2 discs, LR and HR);
• 2 glass vials with caps;
• 1 mirror.

Note: Any damaged or defective item must be returned in

its original packing materials.

HI 3864

Phenols

Test Kit

ISTR3864 11/99 PRINTED IN ITALY

Phenols are widely used in pharmaceuticals, dyes and indica-

tors and as general disinfectants. They may occur in household

and industrial wastewaters and in natural waters; they can

also enter potable water supplies and chlorination of such

waters results in malodorous chlorophenol products that are

detectable from 0.001 mg/L (1 ppb). The HANNA colorimetric

method determines phenol and all ortho and para substituted

phenols. Since substitution generally lowers the response, the

readable value obtained by this method is the minimum

concentration of phenolic compounds present. Natural water

usually contains less than 0.001 mg/L (ppb) of phenols, but

sometimes values up to 0.02 mg/L (20 ppb) also occur.
Since the concentration of phenols in wastewater is generally

subjected to biological and chemical degradation, preserve

samples in the refrigerator and analyze within 4 hours after

collection.
Note: mg/L is equivalent to ppm (parts per million), ppb are

parts per billion.

SPECIFICATIONS

SIGNIFICANCE AND USE

CHEMICAL REACTION

READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
• Verify if the mirror and the LR (0÷1 mg/L) disc are

already pre-installed in the checker. If not, install both
as shown in the figure.

INSTRUCTIONS

Phenolic compounds react to form a yellow to orange dye with

4-amino antipyrine in alkaline solution buffered at pH about

10. The absorbance of this colored product is proportional to

the concentration of phenols present in the aqueous sample.

• Fill each glass vial with 10 mL of

sample, up to the mark.

• Insert one of them into the left hand opening of the

checker disc. This is the blank.

• Add to the other

glass vial 1
packet of HI
3864A-0 Re-

agent. Replace
the cap and
mix.

• Add 1 packet of HI 3864B-0 Reagent.
• Start the waiting time of 10 minutes.

Replace the cap and
shake the vial until the
powder has totally dis-
solved. The solution will
turn a color yellow to or-

ange. This is the reacted
sample.

• Remove the cap and in-

sert the reacted sample
into the right hand open-
ing of the checker disc.

• Hold the checker disc so that

a light source illuminates the
samples from the top.

• After the 10 minutes have

passed, rotate the disc while looking at the color test

windows and stop when you find the color match. Read

Range

0 to 1.00 mg/L (ppm) as Phenols LR

0.5 to 5.0 mg/L (ppm) as Phenols HR

Smallest Increment

0.02 mg/L (ppm) Phenols LR

0.1 mg/L (ppm) Phenols HR

Analysis Method

Colorimetric

Sample Size

10 mL

Number of Tests

100

Case Dimensions

235x175x115 mm (9.2x6.9x4.5")

Shipping Weight

573 g (20.2 oz.)

the value in the result window and record it in mg/L (or

ppm) of Phenols.

• If the color of the sample is too intense to make a color

match with the mirror installed, then remove from the
checker disc:

i. the glass vials
ii. the LR disc
iii. the mirror and its holder.

• Install the HR (0.5÷5 mg/

L) disc and replace the glass
vials in the checker disc.

• Hold the checker disc so that a

light source illuminates the
samples from the back windows.

• Rotate the disc while looking at

the color test windows and stop
when you find the color match.
Read the value in the result
window and record it in mg/L (or ppm) of Phenols.

For best results: Intensely colored samples will make the color

matching determination difficult and they should be
adequately treated before performing the test. Sus-
pended matter in large amounts should be removed
by prior filtration.

Caution:

Ultraviolet radiation may cause fading of colors.

When not in use, keep the disc protected from light,
in a cool and dry place.

The chemicals contained in this kit may be hazardous if
improperly handled. Read Health and Safety Data Sheet
before performing this test.

HEALTH AND SAFETY

REFERENCES

Standard Methods for the Examination of Water and Waste-

water, 18

edition, 1992

10 mL