GE RPN1607 用户手册

5

using 17 pmol/25 ng DNA of 
[

α–

32

P] labelled nucleotides, 

specific activity 3000 Ci/mmol 
(codes PB 10204-7) and 
RPN 1606/1607 are tested 
using 17 pmol/25 ng DNA of 
[

α–

32

P]dCTP, 3000 Ci/mmol 

(code PB 10205). Incorporations 
greater than 55% are achieved 
after 10 minutes incubation 
at 37°C, as assayed by thin-
layer chromatography on PEI 
cellulose in 1.25 M KH

2

PO

4

. 

PH3.4.

In addition components of the 
kits are checked for identity by 
HPLC and the DNA solutions 
for concentration by UV 
spectrophotometry.