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CNBr-activated
Sepharose 4 Fast Flow

The preparation and use of affinity chromatography media by coupling biospecific 
ligands to CNBr-activated matrices is a widely used, successful and well-documented 
technique. 
CNBr-activated Sepharose

TM

 4 Fast Flow is a pre-activated  affinity matrix that 

combines the advantages of CNBr coupling with the high flow and stability 
characteristics of Sepharose 4 Fast Flow. In our experience, the CNBr coupling 
technique has a well-proven track record for the purification of therapeutic 
proteins. This, plus the performance of the matrix at large scale, makes the use of 
CNBr-activated Sepharose 4 Fast Flow particularly attractive for manufacturing 
applications in the biopharmaceutical industry. Furthermore, the medium is a 
member of the BioProcess

TM

 media. BioProcess media are developed and supported 

for production scale chromatography. All BioProcess media are produced with 
validated methods and are tested to meet manufacturing requirements. Secure 
ordering and delivery routines give a reliable supply of media for production 
scale. Regulatory Support Files (RSF) are available to assist process validation and 
submissions to regulatory authorities.
To ensure best performance and trouble-free operation, please read these 
instructions before using CNBr-activated Sepharose 4 Fast Flow.