Trustin Technology SAC2 SAC2-B 用户手册

2.0 METHODOLOGY

2.1 Development of a Standardised Inoculation Procedure

It was considered important to ensure keyboards were contaminated in a way that replicated the "worst
case scenario" likely to be encountered within the NHS and allowed for the different sanitisation
procedures to be compared. Each keyboard was therefore contaminated with high levels of
Staphylococcus Aureus in areas difficult to disinfect. This was performed using the following procedure:

1. A culture of Staphylococcus Aureus was grown under standard conditions in a media containing solids

to mimic soiling.

2. Using clean keyboards the 10 pre-selected keys (C, 5/%, U, F12, 9/pgUp, arrow down key, Delete, Esc,

far left small shift, @) were removed. 0.1mls of the Staphylococcus Aureus culture was inoculated into
each key recess and the keys replaced.

3. The keyboards were left at ambient room temperature for one hour.

4. After the one hour storage the 10 keys were removed and the numbers of remaining Staphylococcus

Aureus were determined by swabbing. 

2.2 Evaluation of Sanitisation Treatments

1. Seven clean keyboards were inoculated with staphylococcus aureus as per point 3 above.

2. After one-hour storage the keyboards were treated for 30 seconds with one of the treatments as listed

in Appendix 1. 

3. Treatment was carried out by preparing 10 litres of a cleaning solution; washing by immersing and

agitating the keyboard (by moving about and pressing the keys) and standing without rinsing or drying
for 2 minutes. 

4. Treatment in 10 litres of sterile water was also carried out as a control and to differentiate between

removal of the bacteria by chemical disinfection and removal by physical washing.

5. The levels of remaining Staphylococcus Aureus were determined as per point 4 above.

6. The percentage kill for each treatment was calculated using the baseline levels of Staphylococcus

Aureus as determined under stage 1.

LABORATORY REPORT

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PILL

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EAL KEYBOARDS

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