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EPA Protocol Calibration 
T400 Ozone Analyzer Operator’s Manual 
210 
 
10.2. LEVEL 1 CALIBRATIONS VERSUS LEVEL 2 CHECKS 
All monitoring instruments are subject to some drift and variation in internal parameters 
and cannot be expected to maintain accurate calibration over long periods of time the 
EPA requires a schedule of periodic checks of the analyzer’s calibration be 
implemented.  Zero and span checks must be used to document that the data remains 
within required limits.  These checks are also used in data reduction and system 
validation. 
A Level 1 Span check is used to document that the T400 is within control limits and 
must be conducted every 2 weeks.  A Level 2 Span Check is to be conducted between 
the Level 1 Checks on a schedule to be determined by the user.  
LEVEL 1 ZERO AND SPAN CALIBRATION (Section 12 of Q.A. Handbook)
11
 
A Level 1 zero and span calibration is a simplified, two-point analyzer calibration used when analyzer linearity 
does not need to be checked or verified.  (Sometimes when no adjustments are made to the analyzer, the 
Level 1 calibration may be called a zero/span check, in which case it must not be confused with a Level 2 
zero/span check.)  Since most analyzers have a reliably linear or near-linear output response with 
concentration, they can be adequately calibrated with only two concentration standards (two-point 
concentration).  Furthermore, one of the standards may be zero concentration, which is relatively easily 
obtained and need not be certified.  Hence, only one certified concentration standard is needed for the two-
point (Level 1) zero and span calibration.  Although lacking the advantages of the multipoint calibration, the 
two-point zero and span calibration--because of its simplicity--can be (and should be) carried out much more 
frequently.  Also, two-point calibrations are easily automated.  Frequency checks or updating of the 
calibration relationship with a two-point zero and span calibration improves the quality of the monitoring data 
by helping to keep the calibration relationship more closely matched to any changes (drifts) in the analyzer 
response. 
LEVEL 2 ZERO AND SPAN CHECK (Section 12 of Q.A. Handbook)
11
 
A Level 2 zero and span check is an "unofficial" check of an analyzer's response.  It may include dynamic 
checks made with uncertified test concentrations, artificial stimulation of the analyzer's detector, electronic 
or other types of checks of a portion of the analyzer, etc. 
Level 2 zero and span checks are not to be used as a basis for analyzer zero or span adjustments, 
calibration updates, or adjustment of ambient data.  They are intended as quick, convenient checks to be 
used between zero and span calibrations to check for possible analyzer malfunction or calibration drift.  
Whenever a Level 2 zero or span check indicates a possible calibration problem, a Level 1 zero and span 
(or multipoint) calibration should be carried out before any corrective action is taken. 
If a Level 2 zero and span check is to be used in the quality control program, a "reference response" for the 
check should be obtained immediately following a zero and span (or multipoint) calibration while the 
analyzer's calibration is accurately known.  Subsequent Level 2 check responses should then be compared 
to the most recent reference response to determine if a change in response has occurred.  For automatic 
Level 2 zero and span checks, the first scheduled check following the calibration should be used for the 
reference response.  It should be kept in mind that any Level 2 check that involves only part of the analyzer's 
system cannot provide information about the portions of the system not checked and therefore cannot be 
used as a verification of the overall analyzer calibration. 
 
06870C DCN6332