Omega Engineering 20 用户手册

Programming Options

There are two options available for creating programs on the CYCLER:

1. Copy and edit one of the pre-written templates.
2. Create a new program from the defaults.

Option 1: Creating programs using templates

There are two pre-written basic PCR templates: a two-step and a three-step template. These can
be copied and modified as required. "In denat" stands for Initial Denaturing.

Other templates available include Ice bucket (incubation at 4ºC), Ligation (incubation at 15ºC) and
an RT-PCR template.

Use the “Down Arrow” key to select an

existing ‘Template’ program from the

menu, e.g. ‘2 Step Template’ or ‘3 Step

Template’.

Press the “Enter” key to accept it.

The Template is already defined and can

be run as it is or easily copied, modified

and saved under a new name.

* * New progr a

2 STEP TEMPLATE 501
3 STE

[ , to select ]

-*New*-

2 STEP
3 STEP
[ , to select ]

2 STEP [2]

3 STEP 3

[ , to select ]

2 STEP TEMPLATE [501]
Run Program
View Program
[ , to select ]

2 STEP [2]

Run Method
View Method
[ , to select ]

[ , to select ]

2 STEP TEMPLATE

Heated Lid 105 ºC
Preheat Lid   on
Pause     off
-  -  -  -  -  - -  -  -  -  -
In denat

94.0 º C 5m00

Hot Start   off
-  -  -  Stage 1 -  -  -  -
Number cycles   30
Step 94.0 ºC 0m30

max

Step 68.0 ºC 1m00

max

- - - - - - - - - - -
Fina Extn 72.0 º C 5m00
Final hold 10.0 ºC

2 STEP

Heated Lid 105 ºC
Preheat Lid   on
Pause     off
-  -  -  -  -  - -  -  -  -  -
In denat

94.0 º C 5m00

Hot Start   off
-  -  -  Stage 1 -  -  -  -
Number cycles   30
Step 94.0 ºC 0m30

max

Step 68.0 ºC 1m00

max

- - - - - - - - - - -
Post run

72.0 º C 5m00

Post hold 10.0 ºC

3 STEP TEMPLATE

Heated Lid    105 º C
Preheat Lid   on
Pause     off
- - - - - - - - -  -  -
In denat

94.0º C 5m00

Hot Start     off
- - - Stage 1 - -  -  -
Number cycles 30
Step 94.0ºC  0m30 max
Step 55.0ºC  0m30 max
Step 72.0ºC  0m30 max
- - - - - - - - -  -  -
Final Extn 72.0º C 5m00
Final hold    10.0 º C

3 STEP

Heated Lid    105 º C
Preheat Lid   on
Pause     off
- - - - - - - - -  -  -
In denat

94.0º C 5m00

Hot Start     off
- - - Stage 1 - -  -  -
Number cycles 30
Step 94.0ºC  0m30 max
Step 55.0ºC  0m30 max
Step 72.0ºC  0m30 max
- - - - - - - - -  -  -
Post

run

72.0º C 5m00

Post hold 10.0 º C