Olympus FV300 用户手册

14

Item

Specifications

Laser light 

Visible light laser source

Select from the following laser, to mounted on laser combiner

source

Multi-line Ar laser (458nm, 488nm, 515nm, Total 40mW), Ar laser (488nm,10mW), Kr laser (568nm, 10mW), 
HeNe (G) laser (543nm,1mW), HeNe (R) laser (633nm,10mW), LD405 (405nm, 25mW),  LD440 (440nm, 5.3mW)

Laser combiner

Each laser light path is equipped with a continuously variable neutral density filter or AOTF
All laser lines are combined to apsis along the same fiber optic

Scanning unit

Scanning method

Galvanometer mirror scanners (both X and Y)

Field number

20 (10 with use of LD405 laser)

Pinhole

5-position pinhole turret

Image memory and 

Standard scanning mode: 256 x 256 (0.45s) - 2048 x 2048 (10.835s)

scanning speed

Bi-directional high-speed scanning mode: 512 x 512 (0.25s) (Simultaneous scanning of up to 2 channels)

Image channel

Selectable from 2-channel (fluorescence) or 2-channel (fluorescence) + 1-channel (transmitted light)
3-channel (fluorescence) using virtual channel

Selection of filters according to staining

Manual selection

Scanning modes

XY, XYZ, XYT, XYZT, XZ, XT, XZT, point, Line-t, free line-t, line-z, free line-z, Clip, ZoomIn

Image depth resolution

12-bit (=4096 grey levels)

Zoom

1X-10X 

Z-drive

Step motor/Minimum step 10nm (BX61, BX61WI and IX81 combination), 25nm (other microscope combination)

Microscopes

Upright

BX51, BX61, BX51WI, BX61WI

Inverted (special laser safe frame)

IX81FVSF, IX71FVSF (side port)

External trans-

Transmitted light illumination unit

External halogen light source connected to microscope via fiber cable

mitted light unit

Transmitted light detector

External detector unit with built-in photomultiplier  Connected to microscope frame via fiber cable

Fluorescence illumination unit

Connect to external mercury light source and microscope via fiber cable
Standard equipment of FV300-BX51, FV-300-BX61, FV300-BX51WI, FV300-BX61WI

PC with system control boards

PC-AT compatible machine/OS: Windows XP (English version)/ 1GB memory (can be expanded to a maximum of 4GB)
CPU: Pentium 4, over 2.8GHz, Special I/F board/image capture: PCI bus
Graphic board: G450_Dual 32MB
Hard disk: 80GB 7200rpm_ID (ATA100) with DVD-ROM
Monitor: Two 19” LCD monitors are recommended, each able to display 1280x1024 images in full color (16.77 million colors)
LAN: On board

Fluoview 

Image acquisition

Scanning condition setting: image size, scanning speed, zoom, panning etc.

application 

Real-time image calculation: Kalman filtering, peak integration, 

software

Hardware control

Laser, scanning unit, microscope

Each image display: Single-channel side-by- side, merge, cropping, tiling, series (Z/T) pass and continuous

Image display

LUT: Individual color setting, pseudo-color, Overlay: Lines, text, scale bar, etc

Image processing

Individual filter: Average, Low-pass, High-pass, Sobel, Median, Prewitt, 2D Laplacian, edge enhancement etc.
Calculations: Inter-image, mathematical and logical, 
DIC back ground leveling

Image analysis

Overview of fluorescence intensity within an area, histogram, perimeter measurement for user-assigned area, 
time-lapse measurement , etc.

3D visualization

3D animation, left / right stereo pairs, red / green stereoscopic images and cross section

Others

Graphic-based help, PAPP (Programmable Acquisition Protocol Processor), time course software (optional), 
trigger IN/OUT function (optional), Multi point software (optional)

Power consumption

Microscope (115V 6A/230V 3A), scanning unit+PSU (115V 3.5A/230V 2A), computer & monitor (115V 4.5A/230V 10A), 
Ar laser (115V 10A/230V 5A), Mult-linei Ar laser (115V 10A/230V 5A), Kr laser (230V 20A), 
HeNe laser each (115V 0.4A/230V 0.2A), LD laser (405nm, 440nm: 100V 0.9A/230V 0.5A)

Objective

N.A.

W.D.

DIC

Revolving

prism

nosepiece

MPL5X

0.10

19.60

—

WI-SSNP,

WI-SRE2

UMPLFL10XW

0.30

3.30

U-LDPW10H

WI-SSNP,

WI-SRE2

UMPLFL20XW

0.50

3.30

U-LDPW20H

WI-SSNP,

WI-SRE2

LUMPLFL40XW

0.80

3.30

U-LDPW40H

WI-SSNP,

WI-SRE2

LUMPLFL60XW

0.90 2.00

U-LDPW60H

WI-SSNP,

WI-SRE2

LUMPLFL40XW/IR

0.80

3.30

U-LDPW40H

WI-SSNP,

WI-SRE2

LUMPLFL60XW/IR

0.90

2.00

U-LDPW60H

WI-SSNP,

WI-SRE2

LUMPLFL100XW

1.00

1.50

U-LDPW60H

WI-SSNP,

WI-SRE2

XLUMPLFL20XW

0.95

*

2.00

U-LDPXLU20

WI-SNPXLU

HR

*

Note: These conditions are not met in confocal microscopy

Condenser for BX Condenser for IX

Description

Immersion

Correction ring

U-UCD8A

IX-LWUCDA

optical element

optical element

UPLSAPO 4X

0.16

13

—

UPLSAPO 10X

0.40 3.1

0.17

U-DIC10

IX2-DIC10

normal

UPLAPO 10XO3

0.40 0.24

0.17

Oil

U-DIC10

IX2-DIC10

normal

UPLAPO 10XW3

0.40 0.43

0.17

Water

U-DIC10

IX2-DIC10

normal

UPLSAPO 20X

0.75

0.6

0.17

U-DIC20

IX2-DIC20

normal

UPLAPO 20XO3

0.80

0.19

—

Oil

U-DIC20

IX2-DIC20

normal

UPLSAPO 40X

0.90

0.2

0.11-0.23

_

U-DIC40

IX2-DIC40

normal

UPLFLN 40XO

1.30

0.2

0.17

Oil

U-DIC40

IX2-DIC40

normal

PLAPON 60XO

1.42

0.15

0.17

Oil

U-DIC60

IX2-DIC60

BFP1

UPLSAPO 60XO

1.35

0.15

0.17

Oil

U-DIC60

X2-DIC60

normal

UPLSAPO 60XW

1.20

0.28

0.15-0.2

Water

_

U-DIC60

X2-DIC60

normal

UPLSAPO 100XO

1.40

0.12

0.17

Oil

U-DIC100

X2-DIC100

normal

NA

W.D

Cover glass

thickness

U-DICTS

position