Princeton 4411-0106 User Manual

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58 
PIXIS System Manual 
Version 2.C 
 
Once the target array temperature {Temperature Setpoint} has been set, the software 
controls the camera's cooling circuits to reach set array temperature. On reaching that 
temperature, the control loop locks to that temperature for stable and reproducible 
performance. When temperature lock has been reached (temperature within 0.05°C of set 
value), the current temperature is 
Locked
. The on-screen indication allows easy 
verification of temperature lock.  
The time required to achieve lock can vary over a considerable range, depending on such 
factors as the camera type, CCD array type, ambient temperature, etc. Once lock occurs, 
it is okay to begin focusing. However, you should wait an additional twenty minutes 
before taking quantitative data so that the system has time to achieve optimum thermal 
stability. 
The deepest operating temperature for a system depends on the CCD array size and 
packaging. Refer to Table 5, on page 95, for typical deepest cooling temperatures. 
Note: In WinX, the Detector Temperature dialog will not display temperature 
information while you are acquiring data. 
Dark Charge 
Dark charge (or dark current) is the thermally induced buildup of charge in the CCD over 
time. The statistical noise associated with this charge is known as dark noise. Dark charge 
values vary widely from one CCD array to another and are exponentially temperature 
dependent. In the case of cameras with MPP type arrays, the average dark charge is 
extremely small. However, the dark-charge distribution is such that a significant number 
of pixels may exhibit a much higher dark charge, limiting the maximum practical 
exposure. Dark charge effect is more pronounced in the case of cameras having a non-
MPP array (such as deep-depletion devices). 
With the light into the camera completely blocked, the CCD will collect a dark charge 
pattern, dependent on the exposure time and camera temperature. The longer the 
exposure time and the warmer the camera, the larger and less uniform this background 
will appear. Thus, to minimize dark-charge effects, you should operate with the lowest 
CCD temperature possible. 
Note: Do not be concerned about either the DC level of this background. What you see 
is not noise. It is a fully subtractable bias pattern. Simply acquire and save a dark charge 
"background image" under conditions identical to those used to acquire the "actual" 
image. Subtracting the background image from the actual image will significantly reduce 
dark-charge effects.  
If you observe a sudden change in the baseline signal, there may be excessive humidity in 
the camera vacuum enclosure. Turn off the camera and contact Princeton Instruments 
Customer Support. See page 132 for contact information.   
Saturation 
When signal levels in some part of the image are very high, charge generated in one pixel 
may exceed the "well capacity" of the pixel, spilling over into adjacent pixels in a process 
called "blooming." In this case a shorter exposure is advisable, with signal averaging to 
enhance S/N (Signal-to-Noise ratio) accomplished through the software.  
WARNING!