Leica CM3050 S Benutzerhandbuch
51
Leica CM3050 S – Cryostat
6.
Troubleshooting, applications tips
Problem
Sections tear.
Inconsistent or insufficient
specimen feed.
specimen feed.
Specimen disc cannot be
removed.
removed.
Cryostat inoperational.
Sectioning motor inoperational.
No or insufficient refrigerating
performance.
performance.
Causes
- Temperature too low for the
tissue to be sectioned.
- Knife blunt, dirty, dusty, frosted
or rusty.
- Upper edge of anti-roll plate
damaged.
- Hard particles in the tissue.
- Rear face of knife dirty.
- Microtome was not completely
dry when refrigeration was
switched on; ice build-up inside
the micrometer mechanism.
switched on; ice build-up inside
the micrometer mechanism.
- Microtome defective.
- Specimen disc stuck to quick-
freeze shelf or specimen head
due to moisture on underside
of specimen disc.
due to moisture on underside
of specimen disc.
- Mains plug not properly con-
nected.
- Automatic cut-out has been
triggered.
- Footswitch not connected.
- Automatic cut-out of sectioning
- Automatic cut-out of sectioning
motor has been trig-
gered.
gered.
- Motor defective.
- Stopper not placed properly
into drain in bottom of cryo-
chamber.
chamber.
- Compressor defective.
- Leak in cooling system.
- Leak in cooling system.
Remedies
- Select higher temperature and
wait for the new temperature
to be reached.
to be reached.
- Eliminate cause (--> see details
on individual problems).
- Exchange anti-roll plate.
- If application permits, section
at lower plane.
- Clean.
- Remove microtome from cham-
ber and dry - possibly with hair-
dryer (hairdryer only to be used
outside the cryochamber!) or at
ambient temperature.
dryer (hairdryer only to be used
outside the cryochamber!) or at
ambient temperature.
- Call Technical Service.
- Apply strong alcohol to contact
point or heat the specimen
head.
head.
- Check mains plug connection.
- Reconnect automatic cut-out
(switch instrument back on).
- Connect footswitch.
- Reconnect automatic cut-out
- Reconnect automatic cut-out
(push inwards until it locks in
place).
place).
- Call Technical Service.
- Close drain with stopper.
- Call Technical Service.
- Call Technical Service.
- Call Technical Service.