Leica CM3050 S Manuel D’Utilisation

51

Leica CM3050 S – Cryostat

6.

Troubleshooting, applications tips

Problem

Sections tear.

Inconsistent or insufficient
specimen feed.

Specimen disc cannot be
removed.

Cryostat inoperational.

Sectioning motor inoperational.

No or insufficient refrigerating
performance.

Causes

- Temperature too low for the

tissue to be sectioned.

- Knife blunt, dirty, dusty, frosted

or rusty.

- Upper edge of anti-roll plate

damaged.

- Hard particles in the tissue.

- Rear face of knife dirty.

- Microtome was not completely

dry when refrigeration was
switched on; ice build-up inside
the micrometer mechanism.

- Microtome defective.

- Specimen disc stuck to quick-

freeze shelf or specimen head
due to moisture on underside
of specimen disc.

- Mains plug not properly con-

nected.

- Automatic cut-out has been

triggered.

- Footswitch not connected.
- Automatic cut-out of sectioning

motor has been                    trig-
gered.

- Motor defective.

- Stopper not placed properly

into drain in bottom of cryo-
chamber.

- Compressor defective.
- Leak in cooling system.

Remedies

- Select higher temperature and

wait for the new temperature
to be reached.

- Eliminate cause (--> see details

on individual problems).

- Exchange anti-roll plate.

- If application permits, section

at lower plane.

- Clean.

 - Remove microtome from cham-

ber and dry - possibly with hair-
dryer (hairdryer only to be used
outside the cryochamber!) or at
ambient temperature.

- Call Technical Service.

- Apply strong alcohol to contact

point or heat the specimen
head.

- Check mains plug connection.

- Reconnect automatic cut-out

(switch instrument back on).

- Connect footswitch.
- Reconnect automatic cut-out

(push inwards until it locks in
place).

- Call Technical Service.

- Close drain with stopper.

- Call Technical Service.
- Call Technical Service.