Gardner LCS IV User Manual
71
Standard programs
5.7 Photometry (LCS IV only)
In the photometry mode, photometric measurements can be
carried out as:
carried out as:
•
Single wavelength measurements
•
Multi wavelength measurements
•
Time course
•
Wavelength scans
5.7.1 Single Wavelength (absorbance, concentration and transmittance readings)
The Single Wavelength mode can be used in three ways. For
sample readings at a single wavelength, the instrument can be
programmed to measure the absorbance, % transmittance or
concentration of the analyte.
sample readings at a single wavelength, the instrument can be
programmed to measure the absorbance, % transmittance or
concentration of the analyte.
•
Absorbance readings: The light absorbed by the sample is
measured in absorbance units.
measured in absorbance units.
•
% Transmittance measures the percent of the original light
that passes through the sample and reaches the detector.
that passes through the sample and reaches the detector.
•
Turning on the concentration factor allows selection of a
specific multiplier for converting absorbance readings to
concentration. In a graph of concentration versus the
absorbance, the concentration factor is the slope of the line.
specific multiplier for converting absorbance readings to
concentration. In a graph of concentration versus the
absorbance, the concentration factor is the slope of the line.
5.7.1.1 Set up Single Wavelength mode
1.
In the main menu, press the Single Wavelength menu
option.
option.
2.
Press Options for Parameter Setup.
Table 7 Options for Single Wavelength
Options
Description
More
For further Options
Save icon
Symbol: Store Data, if Instrument Setup > Data Log Setup > Auto Store: Off is selected.
Symbol: Recall Data, if Instrument Setup > Data Log Setup > Auto Store: On is selected.
Symbol: Recall Data, if Instrument Setup > Data Log Setup > Auto Store: On is selected.
% Trans/Abs
To switch to % transmittance, concentration or absorbance readings