Leica CM3050 S User Manual
41
Leica CM 3050 S – Cryostat
5.5.2 Freezing specimens onto specimen
In instruments with specimen cooling, the 90 °
prism, which is inserted into the specimen head,
is an additional option to freeze specimens at
maximum temperature .
prism, which is inserted into the specimen head,
is an additional option to freeze specimens at
maximum temperature .
Installing the 90 ° prism
•
Loosen screw (3) at specimen head.
•
Insert pin (1) of prism completely into recep-
tacle (2) in the specimen head.
Important: the entire rear surface of the
prism must fit tightly against the specimen
head surface!
tacle (2) in the specimen head.
Important: the entire rear surface of the
prism must fit tightly against the specimen
head surface!
•
Retighten screw (3) at specimen head.
The prism now forms a horizontal surface for
quick specimen freezing.
quick specimen freezing.
Freezing specimens with the 90 ° prism
•
Apply sufficient amount of embedding medi-
um onto ambient temperature specimen disc,
add specimen and orient.
um onto ambient temperature specimen disc,
add specimen and orient.
•
Insert specimen disc with specimen into the
receptacle (4) of the prism.
receptacle (4) of the prism.
•
Press
to activate menu item ‘Fast Object
Freezing’ and activate function by pressing
(see chapter 5.3.4 ‘Menu functions’ item 4).
5.
Operation
4
discs with 90 ° prism
(Instruments w/specimen cooling only)
(Instruments w/specimen cooling only)
The specimen head is now automatically cooled
down to the lowest temperature (-50 °C).
down to the lowest temperature (-50 °C).
•
Once the specimen is frozen, remove the
disc from the prism and store it in the quick-
freeze shelf until inserting it into the speci-
men head.
disc from the prism and store it in the quick-
freeze shelf until inserting it into the speci-
men head.
•
Press
again to activate menu item ‘Fast
Object Freezing’ and deactivate the function
by pressing
once again (see chapter
5.3.4 ‘Menu functions’ item 4).
The specimen head temperature now automati-
cally returns to the previously selected set val-
ue.
cally returns to the previously selected set val-
ue.
•
Loosen screw (3) at specimen head and re-
move prism (1).
move prism (1).
•
Store prism inside the cryochamber.
5.5.3 Optional accessories for freezing
Mobile heat extractor
In addition to the stationary heat extractor (part
of standard delivery), a mobile heat extractor is
available as an optional accessory - see Ap-
pendix ‘Ordering information - accessories ):
of standard delivery), a mobile heat extractor is
available as an optional accessory - see Ap-
pendix ‘Ordering information - accessories ):
•
Store heat extractor in chamber to precool.
•
Place onto specimen in quick-freeze shelf.
•
Remove as soon as specimen is frozen.
specimens onto specimen discs
and for removing frozen specimens
and for removing frozen specimens