GE Q HP Manuel D’Utilisation
p. 2
Code No.
Designation
No. supplied
17-1151-01
HiTrap SP HP
5 x 1 ml
17-1152-01
HiTrap SP HP
5 x 5 ml
17-1153-01
HiTrap Q HP
5 x 1 ml
17-1154-01
HiTrap Q HP
5 x 5 ml
Connectorkit
Connectors supplied
Usage
No. supplied
1/16” male/luer female
Connection of syringe to top of
HiTrap
column
1
Tubing connector
Connection of tubing (e.g. Peristaltic
flangeless/M6 female
Pump P1) to bottom of HiTrap column*
1
Tubing connector
Connection of tubing (e.g. Peristaltic
flangeless/M6 male
Pump P1) to top of HiTrap column**
1
Union 1/16” female/
Connection to original FPLC System
M6 male
through bottom of HiTrap column
1
Union M6 female/
Connection to original FPLC System
1/16” male
through top of HiTrap column
1
Stop plug female, 1/16”
Sealing bottom of HiTrap column
2, 5 or 7
* Union 1/16” female/M6 male is also needed.
** Union M6 female/1/16” male is also needed.
** Union M6 female/1/16” male is also needed.
Table of contents
1. Description
3
2. Selection of ion exchanger and conditions
5
3. Operation
9
4. Optimization
12
5. Choice of gradient type
13
6. Determination of binding capacity
15
7. Scaling up
17
8. Storage
17
9. Ordering Information
17