Leica CM3050 S Manuel D’Utilisation
40
Instruction Manual V1.2 - 11/2006
5.5
Daily operation of the instrument
•
Select chamber temperature and, in instru-
ments with specimen cooling, specimen
temperature according to the type of speci-
men to be sectioned (see also chapter 6.3
‘Reference chart for temperature settings’).
ments with specimen cooling, specimen
temperature according to the type of speci-
men to be sectioned (see also chapter 6.3
‘Reference chart for temperature settings’).
•
Do not continue with step 5.5.1 before the
actual chamber / (specimen) temperature
corresponds to the set value. This may take
several hours (e.g. when operating the in-
strument for the first time or when the instru-
ment had been switched off).
actual chamber / (specimen) temperature
corresponds to the set value. This may take
several hours (e.g. when operating the in-
strument for the first time or when the instru-
ment had been switched off).
Prior to operating the instrument ,
please read and observe the fol-
lowing safety instructions:
please read and observe the fol-
lowing safety instructions:
• ‘Integrated safety devices’
(Chapter 2.2) .
• ‘Handling microtome knives
/ blades’
(Chapter 2.3.4)
(Chapter 2.3.4)
• ‘Knife guard /
Handwheel lock’
(Chapter 2.3.5)
(Chapter 2.3.5)
• ’Motorized sectioning’
(Chapter 2.3.6)
• ‘Defrosting
/ handling frozen tissue’
(Chapter 2.3.7)
(Chapter 2.3.7)
• ‘Frozen instrument parts
and accessories’
(Chapter 2.3.8)
(Chapter 2.3.8)
• ‘Infectious / radioactive material’
(Chapter 2.3.9)
5.5.1 Freezing specimens onto specimen
•
Size the specimen.
•
Apply sufficient amount of embedding medi-
um onto ambient temperature specimen
disc.
um onto ambient temperature specimen
disc.
•
Inside the cryochamber, place the specimen
onto the disc and orient.
onto the disc and orient.
•
Place specimen disc with specimen into one
of the 10 openings in the quick-freeze shelf
(see below) and freeze.
of the 10 openings in the quick-freeze shelf
(see below) and freeze.
The freezing process can be sped up further by
using the stationary heat extractor (1). Next to
the low temperature stabilizer (2) (‘Parking
station’), 3 specimen discs (3) can be placed
within its pivoting range.
using the stationary heat extractor (1). Next to
the low temperature stabilizer (2) (‘Parking
station’), 3 specimen discs (3) can be placed
within its pivoting range.
•
Place the heat extractor (1) directly onto the
specimen surface. After approx. 30 seconds
the specimen is completely frozen.
specimen surface. After approx. 30 seconds
the specimen is completely frozen.
1
2
3
discs with quick-freeze shelf and
stationary heat extractor
stationary heat extractor
5.
Operation