Zeiss Axiovert 100 操作ガイド
3D Microscopy
Monitor version
Axiovert 100/135/135 M
28
B 40-022-4 e 09/98
3.3
Adjustment
Use cover slip as an object and focus on it
using the10x objective;
after adjustment, check the setting using the
objective of the highest magnification
after adjustment, check the setting using the
objective of the highest magnification
Check center position of the 3D illumination
shutter in the same way as with the eyepiece
version;
remove camera cable from the MCU II or
from the camera:
see page 21
version;
remove camera cable from the MCU II or
from the camera:
see page 21
Place the required object on the stage and
focus on it
Switch beam path to camera/TV
A 2-dimensional image first appears on the
monitor;
if the image is reversed, turn the camera
around 180°
if the image is reversed, turn the camera
around 180°
Wear polarizing glasses
The image appears in 3 dimensions on the
monitor
monitor
Place required object on the stage,
focus on it, set Köhler illumination and
adjust aperture diaphragm until optimum 3D
contrast is achieved
adjust aperture diaphragm until optimum 3D
contrast is achieved