Руководство По Работе для Zeiss Axiovert 100

3D Microscopy

Monitor version

Axiovert 100/135/135 M

28

B  40-022-4  e    09/98

 Use cover slip as an object and focus on it

using the10x objective;
after adjustment, check the setting using the
objective of the highest magnification

 Check center position of the 3D illumination

shutter in the same way as with the eyepiece
version;
remove camera cable from the MCU II or
from the camera:
see page 21

 Place the required object on the stage and

focus on it

 Switch beam path to camera/TV

 A 2-dimensional image first appears on the

monitor;
if the image is reversed, turn the camera
around 180°

 Wear polarizing glasses

The image appears in 3 dimensions on the
monitor

 Place required object on the stage,

focus on it, set Köhler illumination and
adjust aperture diaphragm until optimum 3D
contrast is achieved