用户手册目录Ultraviolet Crosslinkers1Operating Instructions1and Service Manual11IMPORTANT: Please read these instructions before operating your UVP UV Crosslinker to familiarize yourself with its operation.1181-0112-01 Rev C1CONTENTS21.Introduction122.Important Safety Information123.Description and Specifications124.Operation325.Applications626.Maintenance, Care and Cleaning727.Changing the UV Wavelength828.Calibration Procedure1029.Replacement Parts12210.General Servicing Procedures132Schematic Diagram1921.INTRODUCTION3UVP Crosslinkers offer researchers an instrument to quickly, safely and efficiently expose samples to a controlled amount of ultraviolet radiation. CL-1000 series features a lay-down type drawer while the CX-2000 has a pull-out drawer. Exposure of sample3Crosslinking of DNA or RNA to nitrocellulose, nylon or reinforced nitrocellulose.3PCR sample contamination control.3Photoniking of DNA3Testing RecA function3Rapid site mapping3UV sterilization and sanitization3UV curing3The crosslinkers are designed to measure and control the ultraviolet (UV) radiation within the exposure chamber. A unique UV sensor continually measures the UV energy and automatically adjusts to variations in UV intensity that occurs as the UV tubes a32.IMPORTANT SAFETY INFORMATION3Ultraviolet Crosslinkers are a powerful source of ultraviolet radiation. Even though they are not easily accessible, do not attempt to disengage or override the internal safety interlocks. Exposure to the UV radiation may result. If the UV sources remain33.DESCRIPTION AND SPECIFICATIONS3The dimensions of the CL-1000 Series UV Crosslinkers:3External: 8.75"H x 13.75"D x 15.75"W (22.2 x 34.9 x 40.0cm)3Interior chamber:5"H x 12"D x 10"W (12.7 x 30.5 x 25.4cm)3Weight:15.5 lbs. (7.5 kg)3Ultraviolet sources: 5 x 8 watt UV dual bipin discharge type tubes3CL-1000 Model:254nm UV3CL-1000L Model:365nm UV3CL-1000M Model:302nm UV3CX-2000 Crosslinker 254nm UV3External: 13.7D x 15.6W x 8.75H in. (348 x 396 x 222 mm) Internal Chamber: 11.3D x 11.4W x 3H in. (305 x 254 x 127 mm)3- 1 -34Features4Microprocessor controlled/UV sensor feedback system4Multiple set functions-4preset UV energy exposure4preset UV time exposure4user set UV energy exposure4user set UV time exposure4Maximum UV energy exposure setting of 999,900 microjoules per cm24Maximum UV time exposure setting of 999.9 minutes4Internal safety interlock4Large LED readout4Tactile membrane switch keypad4UV blocking viewing window4Large interior UV exposure chamber4Dual safety fused4Removable power cord4Laydown type door4Electrical4Part No.Nominal Voltage/Hertz/Amp4CL-1000 Shortwave Crosslinker495-0174-01115V/60Hz/0.7A495-0174-02 230V/50Hz/0.7A495-0174-03 100V/50-60Hz/0.8A4CL-1000L Longwave Crosslinker495-0228-01115V/60Hz/0.7A495-0228-02230V/50Hz/0.7A495-0228-03100V/50-60Hz/0.8A44CL-1000M Midrange Crosslinker495-0230-01115V/60hz/0.7A495-0230-02230V/50Hz/0.7A495-0230-03100V/50-60Hz/0.8A4CX-2000 Shortwave Crosslinker495-0339-01115V/60Hz/0.7A495-0339-02230V/50Hz/0.7A495-0339-03 100V/50-60Hz/0.8A4- 2 -44.OPERATION51.Place the crosslinker on a level work surface. Be sure there is enough room in front for the door to open easily.52.Plug the female end of the power cord into the crosslinker.53.Plug the male end of the power cord into a properly grounded electrical outlet. The proper operating voltage of the crosslinker is found on the product information label. Note: For 230V models, or those requiring special power cord connectors, ensure54.Turn the On/Off switch to the ON position. Note: WHEN TURNED ON THE CROSSLINKER DEFAULTS TO THE LAST USED UV EXPOSURE SETTING.55.The last UV exposure setting will now be displayed on the LED and the last function setting will be noted by glowing red spot(s) on the display panel.56.Place your sample requiring exposure into the chamber.57.The UV Crosslinker can be operated on the following settings:5A.Preset ultraviolet energy exposure setting5Push the PRESET and then ENERGY on the tactile to5Push START. After a slight delay to energize the UV tubes, the LED will begin to countdown. The unit automatically stops at the end of the exposure cycle and will beep FIVE times. Exposure is now complete.5NOTE: Though the PRESET UV EXPOSURE SETTING is fa5Push and hold the PRESET on the tactile touchpad until you hear an audible signal then push ENERGY also on the touchpad.5Set your new Preset UV exposure by pushing the numbers on the touchpad.5- 3 -5Remember: THE LED DISPLAY MUST BE MULTIPLIED BY 100 TO OBTAIN THE PROPER EXPOSURE SETTING.5The new setting will appear on the LED display.6Push ENTER on the touchpad.6The new setting is now installed.6B.Preset ultraviolet time exposure6Push PRESET and then TIME on the tactile touchpad. The red light at each position should now be lit and the preprogrammed UV exposure time of 2 minutes should be displayed in the LED.6Push START on the touchpad. After a slight delay to energize the UV tubes, the LED will begin to countdown. NOTE: THE TIME EXPOSURE IS SET IN MINUTES AND TENTHS OF A MINUTE.6The unit will automatically stop at the end of the exposure cycle and will beep five times. Exposure is now complete.6Information: Though the PRESET ULTRAVIOLET TIME EXPOSURE is factory set to 2 minutes, it is possible to change this if necessary, as follows:6Push and hold the PRESET on the touchpad until you hear an audible signal, then push TIME.6Set your new PRESET UV TIME EXPOSURE by pushing the numbers on the tactile touchpad. The new setting will appear on the LED display.6Push ENTER and the new setting will be installed.6C.User set UV energy exposure6Sometimes it may be necessary for you to set your own standards for exposure. This is easily accomplished as follows:6Push the ENERGY on the tactile touchpad then set your energy exposure requirements by pushing the numbers on the touchpad.6The energy exposure settings should now be displayed on the LED in flashing mode.6- 4 -6REMEMBER: YOUR ENERGY EXPOSURE SETTINGS DISPLAYED MUST BE MULTIPLIED BY 100. If settings are correct, push ENTER on the touchpad.6Push START on the touchpad. After a slight delay to energize the UV tubes, The LED will begin to countdown. the unit will stop automatically at the end of the exposure cycle and will beep five times. Exposure is now complete.7D.User set UV time exposure7Setting your own UV time exposure can be achieved as follows:7Push the TIME on the tactile touchpad and then set your requirements by pushing the numbers. Your time exposure settings will be displayed on the LED in flashing mode. REMEMBER THAT YOUR TIME EXPOSURE SETTINGS ARE SET IN MINUTES AND TENTHS OF A MINUTE7Push START.7After a slight delay to energize the UV tubes, the LED will begin to countdown. The unit will stop automatically at the end of the exposure cycle and will beep five times. Exposure is now complete.78.At the end of the exposure cycle, simply open the door and remove your sample.79.Operational Notes:7A.To abort an exposure, press STOP on the tactile touchpad. The LED will display the remaining exposure.7B.To restart an aborted exposure, press START on the touchpad. The exposure will continue from the point at which the exposure was aborted.7C.To reset an aborted exposure, press the RESET. The LED and touchpad will return to the used last setting.7D.The unit will not operate with the door open. Opening the door during a cycle aborts the cycle. Reclosing the door will reset the cycle to the last entered cycle used. To restart, press the START key.7- 5 -75.APPLICATIONS8The Crosslinker is a multi-purpose ultraviolet exposure instrument for use in the laboratory. A wide variety of applications for ultraviolet radiation exist in the laboratory.8A.UV crosslinking of DNA and RNA by covalently binding nucleic acids to transfer membranes nitrocellulose, nylon or nylon-reinforced nitrocellulose membranes after Northern, Southern, slot or dot blotting.8The PRESET ULTRAVIOLET EXPOSURE SETTING is factor8B.Nicking ethidium-bromide stained DNA in Agarose Gels8C.Gene Mapping for creating cleavage-inhibiting thymine dimers8D.Testing RecA function8E.Ultraviolet Sterilization8F.Elimination of PCR contamination8G.UV Curing8H.Fluorescence of materials8Further Applications can be achieved with the Crosslinkers by changing the ultraviolet wavelength (see Section 7 Changing the wavelength).8- 6 -86.MAINTENANCE, CARE AND CLEANING OF THE CROSSLINKER9The UVP UV Crosslinker is built to provide you with trouble-free operation. To ensure correct service:9NOTE: ALWAYS UNPLUG UNIT FROM ELECTRICAL SUPPLY BEFORE CLEANING OR DRYING.91.Wipe ANY water from inside and outside the unit with a soft cloth or sponge.92.Use soap and water with a soft cloth or sponge to clean the unit.93.Do not allow chemicals to remain on unit surfaces.94.Never clean unit with abrasive pads or cleaners.95.Never clean unit with acetone or chloroform.96.Clean the UV sensor regularly with a soft cloth and alcohol.9UVP offers technical support for all of its products. If you have any questions about product use, operation or repair, please contact UVP Customer Service at the following numbers:9Corporate Headquarters:9UVP, Inc.92066 W. 11th Street,9Upland, CA 91786999TOLL FREE USA (800) 452-67889PHONE: (909) 946-31979FAX: (909) 946-35979E-MAIL: info@uvp.com9European Operations:9Ultra-Violet Products Ltd.9Unit 1, Trinity Hall Estate9Nuffield Road9Cambridge CB4 1TG United Kingdom9PHONE: +44(0)1223 4200229FAX:+44(0)1223 4205619E-MAIL: uvp@uvp.co.uk9NOTE: A Returned Goods Authorization (RGA) number must be obtained before returning any item to UVP.9- 7 -97.CHANGING THE UV WAVELENGTH10A UV Crosslinker is purchased with shortwave, longwave or midrange tubes. However, if user requirements and applications change, the Crosslinkers provide you with the unique ability to change the ultraviolet wavelength and recalibrate the UV sensor and10TUBE P/NWAVELENGTHUVP CALIBRATION SENSOR P/N1034-0006-01365 nm Longwave97-0016-02UVX-36 Longwave1034-0042-01302 nm Midrange97-0016-04UVX-31 Midrange1034-0007-01254 nm Shortwave97-0016-01UVX-25 Shortwave10UV Wavelength Calibration Procedure101.After disconnecting the unit from the electrical supply, install the FIVE NEW WAVELENGTH Tubes as directed in Section 9A. (Changing tubes).102.Plug the corresponding UV calibration sensor into the TOP HOLE just inside of the door at the upper right.103.Place the UV calibration sensor in the middle of the floor of the exposure chamber.104.Close the crosslinker door.105.Invoke the calibration mode of the Crosslinker by pushing/holding down STOP on the tactile touchpad and turning the power to the unit ON.106.A sequence of tones will be heard from the Crosslinker and a 180 second count will display on the LED when the calibration procedure has started.107.No calibration occurs during this 180-second period. This period allows a UV tube warm-up and stabilization period.108.Upon completion of the 180-second period, measurements are automatically made with the UV calibration sensor and the crosslinker sensor. These measurements are compared to limits of acceptability and/or if sensor operation is within range.109.If sensor readings are unacceptable, an error code (01, 02, 03 or 04) will flash on the LED Press any key to stop.10- 8 -1010.Check all connections and redo calibration. If the same error message appears, call UVP.1011.The previous calibrated operation of the crosslinker will continue following any unsuccessful recalibration. Replace the new UV wavelength tubes with the previously removed old wavelength tubes.1112.Successful recalibration to the NEW UV wavelength changes the values in the microprocessor and numeric setting value is displayed on the LED. To return to original or another wavelength the proper UV calibration sensor is needed.11- 9 -118. CROSSLINKER CALIBRATION PROCEDURE12Equipment necessary:12Chart recorder12Two probes with banana plug adapters12One radiometer with UVX-25 calibrated sensor12Cable with banana plug and jack adaptor to connect radiometer to chart-recorder12Power source (100V, 115V or 230V)12Procedure:121.Connect the crosslinker to the power source. Do not turn on the unit at this time.122.Connect the testing probes to the chart recorder and to the crosslinkers PCB as follows:12PEN 1 (red) to the right side of R712PEN 2 (blue) to the right side of R612Ground both pens at the lower part of R212Note: while connecting these probes, the switches for both pens on the chart recorder should be on ZERO position to avoid possible damage to the recorder.123.Place the UVX-25 sensor inside the crosslinker at the center and slide it through the upper hole of the inner chassis. Plug the sensor into the PCB's jack.124.Select 5V scale for both pens. Turn the chart recorder on.125.Select a speed of 15mm/min.126.Switch both pens to MEASURE and DOWN positions so they can touch the chart paper.127.Adjust the ZERO position for both pens.128.Press the CHART DRIVE switch.12- 10 -129.Run the Crosslinker in calibration mode by depressing the STOP/RESET button and turning the power switch on at the same time. At this point, both pens should start recording information on paper. Sometimes either pen will record zero information rega1310.During calibration, check the voltage levels on both pens. By adjusting the potentiometers on the PBC, adjust the values for U4 and U5. Depending on the voltage used, from past units it has been found that the optimum values for these levels are:13VOLTAGERANGE FOR U4RANGE FOR U513115V 3.3-3.5V 2.1-2.3V13220V 3.5-3.7V 1.7-2.0V13100V 2.5-2.7V 2.6-2.9V1311.When the calibration cycle is finished, move both pens to UP and ZERO positions.1312.Unplug the UVX-25 sensor and disconnect the probe for PEN 2.1313.At this point, it is necessary to check the energy levels of the crosslinker to see if the unit was calibrated properly. Slide the UVX-25's cable through the lower hole of the inner chassis and plug it into the radiometer.1314.Connect the radiometer to PEN 2 of the chart recorder.1315.Select 1V scale for PEN 2 and a speed of 60mm/min.1316.Lower both pens, turn the CHART DRIVE switch on, change the pens' switches to MEASURE position and adjust for zero level.1317.Select an energy level of 600 Joules on the crosslinker and press the START button.1318.When the cycle is completed, repeat for 1,200 and 2,000 Joules levels.1319.Calculate the actual amount of energy and compare it with the energy selected. They should be within a range of +/- 10%. If this is not the case, adjustments should be made during a repeat calibration. Repeat all the above procedures until the actual1320.When the three levels of energy are acceptable, disconnect the sensor and the chart recorder from the Crosslinker. At this point, the unit is ready to be tested.13- 11 -139.REPLACEMENT PARTS AND SERVICING PROCEDURES14PART NO.14A.OPERATIONS WITHOUT REMOVING THE TOP COVER5 x 34-0007-0114141414Changing tubes14B.OPERATIONS WITH TOP COVER REMOVED14Replacement of tube holders5 x 36-0003-0114Replacement of starters5 x 53-0001-0214or 53-0132-0114Replacement of starter holders5 x 36-0005-0114Replacement of fuse holders2 x 56-0023-0214Replacement of main connector 55-0105-0114Replacement of main switch53-0134-0114Replacement of UV sensor39-0006-0114Replacement of PCB57-0014-0114Replacement of membrane panel53-0133-0114141414C.OPERATIONS WITH THE INTERNAL CHASSIS ASSEMBLY REMOVED14Replacement of reflector16-0095-0114D.OPERATIONS WITH REFLECTOR REMOVED14Replacement of micro-switch53-0072-0114141414Replacement of ballasts5 x 42-0005-03 100V145 x 42-0005-04 230V145 x 42-0005-01 115V14141414Replacement of magnetic door holders2 x 67-0019-0114E.GENERAL ITEMS14141414Changing fuses2 x 56-0022-0214Changing plastic feet4 x 72-0004-0114- 12 -1410. GENERAL SERVICING PROCEDURES15A. Operations Without Removing The Top Cover15Changing the Tubes15It is recommended to change all FIVE 8-Watt 254nm UV tubes at the same time. The unit does not require recalibration if you are replacing the old sources with new sources of the same wavelength.151.Purchase five tubes from UVP.152.Unplug the unit from the electrical supply.153.Reach up to the inside top of unit and carefully rotate each UV tube a 1/4 to 1/3 turn and pull gently downward.154.After removing all five old tubes, carefully install the five new tubes.155.Dispose of the old UV tubes properly.1515B.Operations With Top Cover Removed15Removal of top cover15Remove the four screws located on the two sides of the unit (2 each side).15Remove the cover, moving in an upwards direction and store safely.15All of the operations below are described as taking place within the unit unless otherwise stated.15Replacement of tube holders151.Open front door on unit.152.Locate tube holders to be changed. (These will be seen at the front and back of the internal chassis assembly - 5 at the front and 5 at the back).153.Loosen and remove screw on the tube holder(s). This screw is located in the main chamber-at the top and just in front of the tube holder(s).154.The tube should now be loose.15- 13 -155.Cut all of the cable ties coming from the tube holder following the two wires from the tube holder to where it terminates. Note: there will be two different termination points for each tube holder. Once this has been located, cut the wires (marking th166.The tube holder(s) can now be removed by pushing the holder into the main chamber and feeding the wires through the cut-out.167.Replace tube holder(s). (Part number 36-0003-01)168.In order to refit the tube holder, reverse the above procedure using butt crimps to reconnect the wires.16Note: If more than one tube holder is to be replaced, the wiring schematic diagram may be of assistance.16Replacement of starters16Locate the starter to be changed. These are positioned in a vertical row on the right hand side of the internal chassis assembly.16Turn starter(s) 30 degrees anti-clockwise and then pull out of the holder.16Reverse above procedure with recommended starter.1616Replacement of starter holder16Locate the starter holder to be changed. It is located on the right hand side of the internal chassis assembly.16Lever off the starter holder using a flat bladed screwdriver. The holder should now be loose.16Replace unit (part number 36-0005-01). This is achieved by first loosening the internal reflector which is held in place with eight screws. The screws can be located in the chamber of the crosslinker - two on the left, two on the right and four on the16To replace the starter holder first push the center of the two black spigots, which hold the starter holder (x2) away from you i.e. towards the crosslinker chamber. As the reflector has been loosened, this should be done fairly easily with a small, fla16The starter can now be relocated.16- 14 -16To secure the starter holder, place the holder in your right hand and with your left hand in the chamber, press the now loosened reflector adjacent to the holder inwards. This will push the black spigots inwards thereby securing the starter holder.17Re-fit the reflector.17Replacement of fuse holders17These are located at the back of the unit just above the mains connector. There are two fuses in separate holders. Replacement is the same for both.17Cut back the heat shrink on the two wires.17Unsolder both connections and pull from the holder.17Unscrew the nylon nut and push the holder out through the back of the unit.17To replace-reverse the above procedure.17Replacement of main connector17This is located at the back of the unit below the fuse holders.17Cut back the heat shrink on the three wires.17Unsolder the three connections and pull from connector.17Release main connector by holding the nuts with a spanner and then on the outside back of the unit, release the two screws one at a time (using a screwdriver). The main connector should now be loose.17To replace-reverse the above procedure.17Replacement of main switch17This is located on the front panel below the membrane pad.17Remove the wires noting their position.17Remove the switch by pushing it out from the back towards the rear of the front panel.17Push in the new switch and re-connect.17- 15 -17Replacement of UV sensor18UVP recommends returning the Crosslinker to the UVP factory for service. This is located on the right hand side of the internal chassis assembly at the back near the base.18Unplug the sensor connector from the PCB board marked J2.18Release the sensor by removing the location screw with a screwdriver and holding the nut inside the main chamber with a spanner. Pull sensor clear.18For replacement, reverse the above procedure.18Note: The crosslinker will now need recalibration-see section 8.18Replacement of PCB18The main PCB is located on the right of the unit directly behind the front membrane panel.18Disconnect the ribbon connector J4. See wiring diagram for position.18Unscrew the four screws located at the top and bottom. Note: if the membrane panel needs to be replaced, it should be replaced now. See next paragraph.18For replacement-reverse the above procedure.18Replacement of membrane panel18Located on the front panel.18Carry out the above procedure-replacement of PCB.18Peel off membrane after first marking the position with a pencil, and feed out the ribbon cable.18Replace membrane pad.18Note: Marking the position of the membrane will ensure that the new part will be in the exact position as fitted from the factory.18- 16 -18C. Operations With The Internal Chassis Assembly Removed19Removal of the assembly19Release the wire to the micro switch located on the bottom left internal chassis assembly.19Release the main power cable to ballasts - black wire with connector (color red).19Release all eight nuts positioned four on top of the unit, two sets on either side of the unit. See diagram for positions.19Slide unit out from the main frame.19Replacement of reflector19The reflector is located inside of the internal chassis assembly.19Release the eight screws-four on the top and two on either side of the unit.19Pull the reflector out towards the front of the unit. Note: for cleaning the reflector, refer to Section 6 for maintenance/care/cleaning of the crosslinker.19For replacement-reverse the above procedure.19D. Operations With The Reflector Removed19Replacement of ballasts19The ballasts are fitted to the right hand side of the internal chassis assembly.191.Select the ballast(s) which need to be replaced.192.Cut the tie wraps to locate the ballast electrical connectors.193.Cut these wires and mark positions.194.Re-fit new ballasts using M4 posidrive screws with shake proof and plain washers with a nut as fitted on the diagram.19- 17 -19E. General Items20Changing fuses20The fuses are located on the back panel and are marked with a self-adhesive sticker.201. Turn fuse cap 15 degrees anti-clockwise.202. Pull fuse and cap from the holder.203. Pull fuse from the cap.204. Replace the fuse.205. Replace fuse cap and fuse into holder and turn 15 degrees clockwise.20Changing plastic feet20The feet are fitted to the bottom of the cabinet. They are removed by unscrewing the screw mounted inside each foot.20- 18 -20Schematic Wiring Diagram2121- 19 -21Internet: www.uvp.com22Corporate Headquarters: UVP, Inc.222066 W. 11th Street22Upland, CA 9178622Tel: (800)452-6788 or (909)946-319722Fax: (909)946-3597 / E-Mail: info@uvp.com22European Operations:22Ultra-Violet Products Ltd.22Unit 1, Trinity Hall Estate, Nuffield Road22Cambridge CB4 1TG United Kingdom22Tel: +44(0)1223 42002222Fax: +44(0)1223 420561 / E-Mail: uvp@uvp.co.uk22文件大小: 580.3 KB页数: 22Language: English打开用户手册