Diagnostic Grifols S.A. 215550 User Manual
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Figure 9.24. Graphic Description of the “Time to Absorbance Increment” Algorithm
9.2.2.3 Algorithms for Chromogenic Tests
The algorithms used in chromogenic tests calculate the rate (slope) of colour formation (mE/min) or
the absolute absorbance increase (mE) due to the enzymatic activity (hydrolysis of a chromogenic
substrate).
substrate).
Two Slopes (30-90)
It measures the absorbance increase (due to colour formation) during 1 minute and the result is
reported in mE/min (slope of the reaction curve).
It is called “Two Slopes (30-90)” because it calculates the slope of 2 stretches (30-60 and 60-90) and
it checks the linearity between them.
reported in mE/min (slope of the reaction curve).
It is called “Two Slopes (30-90)” because it calculates the slope of 2 stretches (30-60 and 60-90) and
it checks the linearity between them.
Figure 9.25. Graphic Description of the “Two Slopes (30-90)” Algorithm